Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201108
Type: Artigo de periódico
Title: The Gastroprotective Effect Of Menthol: Involvement Of Anti-apoptotic, Antioxidant And Anti-inflammatory Activities.
Author: Rozza, Ariane Leite
Meira de Faria, Felipe
Souza Brito, Alba Regina
Pellizzon, Cláudia Helena
Abstract: The aim of this research was to investigate the anti-apoptotic, antioxidant and anti-inflammatory properties of menthol against ethanol-induced gastric ulcers in rats. Wistar rats were orally treated with vehicle, carbenoxolone (100 mg/kg) or menthol (50 mg/kg) and then treated with ethanol to induce gastric ulcers. After euthanasia, stomach samples were prepared for histological slides and biochemical analyses. Immunohistochemical analyses of the cytoprotective and anti-apoptotic heat-shock protein-70 (HSP-70) and the apoptotic Bax protein were performed. The neutrophils were manually counted. The activity of the myeloperoxidase (MPO) was measured. To determine the level of antioxidant functions, the levels of glutathione (GSH), glutathione peroxidase (GSH-Px), glutathione reductase (GR) and superoxide dismutase (SOD) were measured using ELISA. The levels of the pro-inflammatory cytokines tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) and the anti-inflammatory cytokine interleukin-10 (IL-10) were assessed using ELISA kits. The menthol treated group presented 92% gastroprotection compared to the vehicle-treated group. An increased immunolabeled area was observed for HSP-70, and a decreased immunolabeled area was observed for the Bax protein in the menthol treated group. Menthol treatment induced a decrease in the activity of MPO and SOD, and the protein levels of GSH, GSH-Px and GR were increased. There was also a decrease in the levels of TNF-α and IL-6 and an increase in the level of IL-10. In conclusion, oral treatment with menthol displayed a gastroprotective activity through anti-apoptotic, antixidant and anti-inflammatory mechanisms.
Subject: Animals
Anti-inflammatory Agents
Antioxidants
Apoptosis
Ethanol
Gastric Mucosa
Glutathione
Glutathione Peroxidase
Glutathione Reductase
Hsp70 Heat-shock Proteins
Interleukin-10
Interleukin-6
Male
Menthol
Neutrophils
Peroxidase
Rats
Rats, Wistar
Stomach Ulcer
Superoxide Dismutase
Tumor Necrosis Factor-alpha
Bcl-2-associated X Protein
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0086686
Address: http://www.ncbi.nlm.nih.gov/pubmed/24466200
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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