Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201097
Type: Artigo de periódico
Title: A Model System To Study The Lignification Process In Eucalyptus Globulus.
Author: Araújo, Pedro
Cesarino, Igor
Mayer, Juliana Lischka Sampaio
Ferrari, Ilse Fernanda
Kiyota, Eduardo
Sawaya, Alexandra Christine Helena Frankland
Paes Leme, Adriana Franco
Mazzafera, Paulo
Abstract: Recalcitrance of plant biomass is closely related to the presence of the phenolic heteropolymer lignin in secondary cell walls, which has a negative effect on forage digestibility, biomass-to-biofuels conversion and chemical pulping. The genus Eucalyptus is the main source of wood for pulp and paper industry. However, when compared to model plants such as Arabidopsis thaliana and poplar, relatively little is known about lignin biosynthesis in Eucalyptus and only a few genes were functionally characterized. An efficient, fast and inexpensive in vitro system was developed to study lignification in Eucalyptus globulus and to evaluate the potential role of candidate genes in this biological process. Seedlings were grown in four different conditions, in the presence or absence of light and with or without sucrose in the growth medium, and several aspects of lignin metabolism were evaluated. Our results showed that light and, to a lesser extent, sucrose induced lignin biosynthesis, which was followed by changes in S/G ratio, lignin oligomers accumulation and gene expression. In addition, higher total peroxidase activity and differential isoperoxidase profile were observed when seedlings were grown in the presence of light and sucrose. Peptide sequencing allowed the identification of differentially expressed peroxidases, which can be considered potential candidate class III peroxidases involved in lignin polymerization in E. globulus.
Rights: fechado
Identifier DOI: 10.1111/ppl.12152
Address: http://www.ncbi.nlm.nih.gov/pubmed/24444279
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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