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Type: Artigo de periódico
Title: Development Of A Non-viral Gene Delivery Vector Based On The Dynein Light Chain Rp3 And The Tat Peptide.
Author: Favaro, M T P
de Toledo, M A S
Alves, R F
Santos, C A
Beloti, L L
Janissen, R
de la Torre, L G
Souza, A P
Azzoni, A R
Abstract: Gene therapy and DNA vaccination trials are limited by the lack of gene delivery vectors that combine efficiency and safety. Hence, the development of modular recombinant proteins able to mimic mechanisms used by viruses for intracellular trafficking and nuclear delivery is an important strategy. We designed a modular protein (named T-Rp3) composed of the recombinant human dynein light chain Rp3 fused to an N-terminal DNA-binding domain and a C-terminal membrane active peptide, TAT. The T-Rp3 protein was successfully expressed in Escherichia coli and interacted with the dynein intermediate chain in vitro. It was also proven to efficiently interact and condense plasmid DNA, forming a stable, small (∼100nm) and positively charged (+28.6mV) complex. Transfection of HeLa cells using T-Rp3 revealed that the vector is highly dependent on microtubule polarization, being 400 times more efficient than protamine, and only 13 times less efficient than Lipofectamine 2000™, but with a lower cytotoxicity. Confocal laser scanning microcopy studies revealed perinuclear accumulation of the vector, most likely as a result of transport via microtubules. This study contributes to the development of more efficient and less cytotoxic proteins for non-viral gene delivery.
Subject: Cytoplasmic Dyneins
Gene Products, Tat
Gene Transfer Techniques
Genetic Vectors
Hela Cells
Microscopy, Confocal
Molecular Mimicry
Recombinant Proteins
Dynein Light Chain Rp3
Gene Delivery
Non-viral Protein Vectors
Citation: Journal Of Biotechnology. v. 173, p. 10-8, 2014-Mar.
Rights: fechado
Identifier DOI: 10.1016/j.jbiotec.2014.01.001
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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