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Type: Artigo de periódico
Title: P-i Class Metalloproteinase From Bothrops Moojeni Venom Is A Post-proline Cleaving Peptidase With Kininogenase Activity: Insights Into Substrate Selectivity And Kinetic Behavior.
Author: Okamoto, Débora N
Kondo, Marcia Y
Oliveira, Lilian C G
Honorato, Rodrigo V
Zanphorlin, Leticia M
Coronado, Monika A
Araújo, Mariana S
da Motta, Guacyara
Veronez, Camila L
Andrade, Sheila S
Oliveira, Paulo S L
Arni, Raghuvir K
Cintra, Adelia C O
Sampaio, Suely V
Juliano, Maria A
Juliano, Luiz
Murakami, Mário T
Gouvea, Iuri E
Abstract: Snake venom metalloproteinases (SVMPs) belonging to P-I class are able to hydrolyze extracellular matrix proteins and coagulation factors triggering local and systemic reactions by multiple molecular mechanisms that are not fully understood. BmooMPα-I, a P-I class SMVP from Bothrops moojeni venom, was active upon neuro- and vaso-active peptides including angiotensin I, bradykinin, neurotensin, oxytocin and substance P. Interestingly, BmooMPα-I showed a strong bias towards hydrolysis after proline residues, which is unusual for most of characterized peptidases. Moreover, the enzyme showed kininogenase activity similar to that observed in plasma and cells by kallikrein. FRET peptide assays indicated a relative promiscuity at its S2-S'2 subsites, with proline determining the scissile bond. This unusual post-proline cleaving activity was confirmed by the efficient hydrolysis of the synthetic combinatorial library MCA-GXXPXXQ-EDDnp, described as resistant for canonical peptidases, only after Pro residues. Structural analysis of the tripeptide LPL complexed with BmooMPα-I, generated by molecular dynamics simulations, assisted in defining the subsites and provided the structural basis for subsite preferences such as the restriction of basic residues at the S2 subsite due to repulsive electrostatic effects and the steric impediment for large aliphatic or aromatic side chains at the S1 subsite. These new functional and structural findings provided a further understanding of the molecular mechanisms governing the physiological effects of this important class of enzymes in envenomation process.
Subject: Amino Acid Sequence
Crotalid Venoms
Molecular Dynamics Simulation
Serine Endopeptidases
Substrate Specificity
Fret Peptides
Kininogenase Activity
Molecular Dynamics Simulations
Snake Venom Metalloproteinase
Substrate Specificity
Citation: Biochimica Et Biophysica Acta. v. 1844, n. 3, p. 545-52, 2014-Mar.
Rights: fechado
Identifier DOI: 10.1016/j.bbapap.2013.12.014
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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