Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201050
Type: Artigo de periódico
Title: Lipid Characterization Of Embryo Zones By Silica Plate Laser Desorption Ionization Mass Spectrometry Imaging (sp-ldi-msi).
Author: Ferreira, Mônica S
de Oliveira, Diogo N
Gonçalves, Roseli F
Catharino, Rodrigo R
Abstract: Lipid pathways play important biological roles in mammalian embryology, directing early developmental pathways to differentiation. Phospholipids and triglycerides, among others, are the main composing lipids of zona pellucida in several embryo species. Lipid analysis in embryos by mass spectrometry usually requires sample preparation and/or matrix application. This novel approach using silica plate laser desorption/ionization mass spectrometry imaging (SP-LDI-MSI) allows direct single-cell imaging and embryo region discrimination with no matrix coating. Its application is herein described for two- and eight-cell embryos. Lipid biomarkers for blastomere and intact zona pellucida are reported and corroborated by both fragmentation reactions (MS/MS) and images. Results obtained in this work are understood to be of great use for further developments on in vitro bovine fertilization. Since much of the processes can be monitored by characteristic biomarkers, it is now possible to precisely identify cell division errors during early embryo stages, as well as evaluate pre-implantation conditions.
Subject: Animals
Biological Markers
Cattle
Embryo, Mammalian
Lipids
Principal Component Analysis
Silicon Dioxide
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
2,5-dihydroxybenzoic Acid
Biomarkers
Cer
Dhb
Direct Analysis
Maldi-ms
Metabolic Fingerprinting
Pa
Pca
Pe
Pl
Principal Component Analysis
Sp-ldi-msi
Single-cell
Tlc
Zp
Ceramide
Isok
Isoketals
Matrix-assisted Laser Desorption/ionization Mass Spectrometry
Phosphatidic Acid
Phosphatidylethanolamine
Phospholipid
Silica Plate Laser Desorption/ionization Mass Spectrometry Imaging
Thin-layer Chromatography
Zona Pellucida
Rights: fechado
Identifier DOI: 10.1016/j.aca.2013.11.033
Address: http://www.ncbi.nlm.nih.gov/pubmed/24356225
Date Issue: 2014
Appears in Collections:Unicamp - Artigos e Outros Documentos

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