Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201046
Type: Artigo
Title: Short and long-term antinutritional effect of the trypsin Inhibitor ApTI for biological control of sugarcane borer
Author: Silva, Desireé S. da
Oliveira, Caio F. R. de
Parra, José R. P.
Marangoni, Sergio
Macedo, Maria L. R.
Abstract: Plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promising alternative for the biological control of pests. In this work, we tested the anti-insect activity of Adenanthera pavonina trypsin inhibitor (ApTI) against Diatraea saccharalis larvae, a major insect pest in sugarcane. The addition of 0.1% ApTI in short-term assays resulted in 87% and 63% decreased trypsin and chymotrypsin activities respectively. ApTI was not digested after 60h incubation with D. saccharalis midgut proteases. The chronic effects of ApTI on F0 and F1 generations of D. saccharalis were also analyzed. The larvae from the F0 generation showed 55% and 21% decreased larval and pupal viability, respectively. ApTI-fed larvae from the F1 generation showed a decrease of 33% in survival rate and 23% in the average larval weight. Moreover, ApTI treatment reduced trypsin and chymotrypsin activities in F1 larvae. Thus, the anti-insect effects of ApTI on consecutive generations (F0 and F1) of D. saccharalis larvae demonstrate its potential for long-term control of this pest.
Plant-derived trypsin inhibitors have been shown to have potent anti-insect effects and are a promisingalternative for the biological control of pests. In this work, we tested the anti-insect activity ofAdenan-thera pavonina trypsin inhibitor (ApTI) against Diatraea saccharalis larvae, a major insect pest in sugar-cane. The addition of 0.1% ApTI in short-term assays resulted in 87% and 63% decreased trypsin andchymotrypsin activities respectively. ApTI was not digested after 60 h incubation withD. saccharalismid-gut proteases. The chronic effects of ApTI on F0 and F1 generations of D. saccharalis were also analyzed.The larvae from the F0 generation showed 55% and 21% decreased larval and pupal viability, respectively.ApTI-fed larvae from the F1 generation showed a decrease of 33% in survival rate and 23% in the averagelarval weight. Moreover, ApTI treatment reduced trypsin and chymotrypsin activities in F1 larvae. Thus,the anti-insect effects of ApTI on consecutive generations (F0 and F1) of D. saccharalis larvae demonstrateits potential for long-term control of this pest.
Subject: Adenanthera pavonina
Controle biológico
Broca-da-cana-de-açúcar
Relação inseto-planta
Inibidores da tripsina
Country: Reino Unido
Editor: Elsevier
Citation: Journal Of Insect Physiology. v. 61, p. 1-7, 2014-Feb.
Rights: fechado
Identifier DOI: 10.1016/j.jinsphys.2013.11.012
Address: https://www.sciencedirect.com/science/article/pii/S0022191013002473
Date Issue: 2014
Appears in Collections:IB - Artigos e Outros Documentos

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