Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201042
Type: Artigo de periódico
Title: Effect Of Low-level Laser Therapy On Odontoblast-like Cells Exposed To Bleaching Agent.
Author: Lima, Adriano Fonseca
Ribeiro, Ana Paula Dias
Basso, Fernanda Gonçalves
Bagnato, Vanderlei Salvador
Hebling, Josimeri
Marchi, Giselle Maria
de Souza Costa, Carlos Alberto
Abstract: The aim of the present study was to evaluate the effect of low-level laser therapy (LLLT) on odontoblast-like MDPC-23 cells exposed to carbamide peroxide (CP 0.01%-2.21 μg/mL of H2O2). The cells were seeded in sterile 24-well plates for 72 h. Eight groups were established according to the exposure or not to the bleaching agents and the laser energy doses tested (0, 4, 10, and 15 J/cm(2)). After exposing the cells to 0.01% CP for 1 h, this bleaching solution was replaced by fresh culture medium. The cells were then irradiated (three sections) with a near-infrared diode laser (InGaAsP-780 ± 3 nm, 40 mW), with intervals of 24 h. The 0.01% CP solution caused statistically significant reductions in cell metabolism and alkaline phosphate (ALP) activity when compared with those of the groups not exposed to the bleaching agent. The LLLT did not modulate cell metabolism; however, the dose of 4 J/cm(2) increased the ALP activity. It was concluded that 0.01% CP reduces the MDPC-23 cell metabolism and ALP activity. The LLLT in the parameters tested did not influence the cell metabolism of the cultured cells; nevertheless, the laser dose of 4 J/cm(2) increases the ALP activity in groups both with and without exposure to the bleaching agent.
Rights: fechado
Identifier DOI: 10.1007/s10103-013-1309-2
Address: http://www.ncbi.nlm.nih.gov/pubmed/23525831
Date Issue: 2014
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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