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Type: Artigo de periódico
Title: Pharmaceutical Analysis In Solids Using Front Face Fluorescence Spectroscopy And Multivariate Calibration With Matrix Correction By Piecewise Direct Standardization.
Author: Alves, Julio Cesar L
Poppi, Ronei J
Abstract: This paper reports the application of piecewise direct standardization (PDS) for matrix correction in front face fluorescence spectroscopy of solids when different excipients are used in a pharmaceutical preparation based on a mixture of acetylsalicylic acid (ASA), paracetamol (acetaminophen) and caffeine. As verified in earlier studies, the use of different excipients and their ratio can cause a displacement, change in fluorescence intensity or band profile. To overcome this important drawback, a standardization strategy was adopted to convert all the excitation-emission fluorescence spectra into those used for model development. An excitation-emission matrix (EEM) for which excitation and emission wavelengths ranging from 265 to 405 nm and 300 to 480 nm, respectively, was used. Excellent results were obtained using unfolded partial least squares (U-PLS), with RMSEP values of 8.2 mg/g, 10.9 mg/g and 2.7 mg/g for ASA, paracetamol and caffeine, respectively, and with relative errors lesser than 5% for the three analytes.
Subject: Acetaminophen
Analgesics, Non-narcotic
Anti-inflammatory Agents, Non-steroidal
Central Nervous System Stimulants
Least-squares Analysis
Spectrometry, Fluorescence
Rights: fechado
Identifier DOI: 10.1016/j.saa.2012.10.074
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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