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http://repositorio.unicamp.br/jspui/handle/REPOSIP/201015
Type: | Artigo de periódico |
Title: | Effects Of Fish Oil Containing Eicosapentaenoic Acid And Docosahexaenoic Acid On Dystrophic Mdx Mice. |
Author: | Fogagnolo Mauricio, Adriana Minatel, Elaine Santo Neto, Humberto Marques, Maria Julia |
Abstract: | In Duchenne muscular dystrophy (DMD) and in the mdx mouse model of DMD, the lack of dystrophin leads to muscle degeneration and inflammation contributes to progression of the disease. In this study, we evaluated the effects of commercially available fish oil containing EPA and docosahexaenoic acid (DHA) on mdx. Mdx mice (14 days old) were treated with fish oil (FDC Vitamins; 0.002 g EPA and 0.001 g DHA) for 16 days by gavage. Control mdx mice received mineral oil (Nujol). Grip strength measurement was used for functional evaluation. The sternomastoid, diaphragm and biceps brachii muscles were removed and processed for histopathology and Western blot analysis. Fish oil decreased creatine kinase and myonecrosis. In all muscles studied, the inflammatory area was significantly reduced after treatment (18.0 ± 3.0% inflammatory area in untreated mdx mice versus 4.0 ± 1% in treated mdx mice). Fish oil protected against the loss of muscle strength. Fish oil significantly reduced the levels of TNF-α and the levels of 4-HNE-protein adducts (30-34% reduction for both) in all muscles studied. Commercially available fish oil may be potentially useful to ameliorate dystrophic progression of skeletal muscles, deserving further clinical trials in DMD patients. |
Subject: | Animals Creatine Kinase Diaphragm Disease Models, Animal Docosahexaenoic Acids Dystrophin Eicosapentaenoic Acid Female Fish Oils Male Mice Mice, Inbred C57bl Mice, Inbred Mdx Muscle Strength Muscle, Skeletal Muscular Dystrophy, Animal Muscular Dystrophy, Duchenne Tumor Necrosis Factor-alpha |
Citation: | Clinical Nutrition (edinburgh, Scotland). v. 32, n. 4, p. 636-42, 2013-Aug. |
Rights: | fechado |
Identifier DOI: | 10.1016/j.clnu.2012.11.013 |
Address: | http://www.ncbi.nlm.nih.gov/pubmed/23218947 |
Date Issue: | 2013 |
Appears in Collections: | Unicamp - Artigos e Outros Documentos |
Files in This Item:
File | Size | Format | |
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pmed_23218947.pdf | 1.4 MB | Adobe PDF | View/Open |
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