Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201013
Type: Artigo de periódico
Title: Cellulitis Lesions In Broiler Chickens Are Induced By Escherichia Coli Vacuolating Factor (ecvf).
Author: Quel, N G
Aragão, A Z B
Salvadori, M R
Farias, A S
Joazeiro, P P
Santos, L M B
Sá, L R M
Ferreira, A J P
Yano, T
Abstract: Escherichia coli Vacuolating Factor (ECVF) is a heat-labile, vacuolating cytotoxin produced by avian pathogenic E. coli (APEC) isolated from avian cellulitis lesions. In this report, we intend to demonstrate that purified ECVF induces the inflammatory process of cellulitis. Our group is the first to demonstrate the effect of ECVF in a histological analysis by in situ inoculation of broiler chickens with purified ECVF. The animals were inoculated with the APEC AC53 and with purified ECVF subcutaneously on their ventral surface (in the sternum region). The histological analysis showed different grades of an acute inflammatory response in the epidermis, dermis and panniculus. An increase in mRNA expression of the proinflammatory cytokine TNF-α was also demonstrated in the inflamed tissue. When ECVF was systemically administered, increased levels of TNF-α and IL-10 were observed in the serum. These results suggest that ECVF plays a key role in the inflammatory process associated with cellulitis that is mainly mediated by TNF-α. In addition, this inflammation can be downregulated by the anti-inflammatory cytokine IL-10.
Subject: Animals
Bacterial Toxins
Cellulitis
Chick Embryo
Chickens
Escherichia Coli
Escherichia Coli Infections
Interleukin-10
Male
Poultry Diseases
Rna, Messenger
Tumor Necrosis Factor-alpha
Rights: fechado
Identifier DOI: 10.1016/j.vetmic.2012.11.007
Address: http://www.ncbi.nlm.nih.gov/pubmed/23206410
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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