Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/201003
Type: Artigo de periódico
Title: Lllt Improves Tendon Healing Through Increase Of Mmp Activity And Collagen Synthesis.
Author: Guerra, Flávia Da Ré
Vieira, Cristiano Pedrozo
Almeida, Marcos Santos
Oliveira, Letícia Prado
de Aro, Andrea Aparecida
Pimentel, Edson Rosa
Abstract: The Achilles tendon has a high incidence of rupture, and the healing process leads to a disorganized extracellular matrix (ECM) with a high rate of injury recurrence. To evaluate the effects of different conditions of low-level laser (LLL) application on partially tenotomized tendons, adult male rats were divided into the following groups: G1, intact; G2, injured; G3, injured + LLL therapy (LLLT; 4 J/cm(2) continuous); G4, injured + LLLT (4 J/cm(2), 20 Hz); G5, injured; G6, injured + LLLT (4 J/cm(2) continuous); and G7, injured + LLLT (4 J/cm(2), 20 Hz until the 7th day and 2 kHz from 8 to 14 days). G2, G3, and G4 were euthanized 8 days after injury, and G5, G6, and G7 were euthanized on the 15th day. The quantification of hydroxyproline (HOPro) and non-collagenous protein (NCP), zymography for matrix metalloproteinase (MMP)-2 and MMP-9, and Western blotting (WB) for collagen types I and III were performed. HOPro levels showed a significant decrease in all groups (except G7) when compared with G1. The NCP level increased in all transected groups. WB for collagen type I showed an increase in G4 and G7. For collagen type III, G4 presented a higher value than G2. Zymography for MMP-2 indicated high values in G4 and G7. MMP-9 increased in both treatment groups euthanized at 8 days, especially in G4. Our results indicate that the pulsed LLLT improved the remodeling of the ECM during the healing process in tendons through activation of MMP-2 and stimulation of collagen synthesis.
Subject: Achilles Tendon
Animals
Collagen
Collagen Type I
Collagen Type Iii
Disease Models, Animal
Hydroxyproline
Laser Therapy, Low-level
Lasers, Semiconductor
Male
Matrix Metalloproteinase 2
Matrix Metalloproteinase 9
Rats
Rats, Wistar
Rupture
Tendon Injuries
Wound Healing
Rights: fechado
Identifier DOI: 10.1007/s10103-012-1236-7
Address: http://www.ncbi.nlm.nih.gov/pubmed/23179310
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_23179310.pdf338.73 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.