Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200990
Type: Artigo de periódico
Title: Development And Characterization Of Microsatellite Markers For The Medicinal Plant Smilax Brasiliensis (smilacaceae) And Related Species.
Author: Martins, Aline R
Abreu, Aluana G
Bajay, Miklos M
Villela, Priscilla M S
Batista, Carlos E A
Monteiro, Mariza
Alves-Pereira, Alessandro
Figueira, Glyn M
Pinheiro, José B
Appezzato-da-Glória, Beatriz
Zucchi, Maria I
Abstract: • A new set of microsatellite or simple sequence repeat (SSR) markers were developed for Smilax brasiliensis, which is popularly known as sarsaparilla and used in folk medicine as a tonic, antirheumatic, and antisyphilitic. Smilax brasiliensis is sold in Brazilian pharmacies, and its origin and effectiveness are not subject to quality control. • Using a protocol for genomic library enrichment, primer pairs were developed for 26 microsatellite loci and validated in 17 accessions of S. brasiliensis. Thirteen loci were polymorphic and four were monomorphic. The primers successfully amplified alleles in the congeners S. campestris, S. cissoides, S. fluminensis, S. goyazana, S. polyantha, S. quinquenervia, S. rufescens, S. subsessiliflora, and S. syphilitica. • The new SSR markers described herein are informative tools for genetic diversity and gene flow studies in S. brasiliensis and several congeners.
Subject: Smilax
Medicinal Plant
Microsatellites
Sarsaparilla
Transferability
Rights: fechado
Identifier DOI: 10.3732/apps.1200507
Address: http://www.ncbi.nlm.nih.gov/pubmed/25202555
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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