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Type: Artigo de periódico
Title: In Vitro And In Vivo Investigation Of The Biological And Mechanical Behaviour Of Resin-modified Glass-ionomer Cement Containing Chlorhexidine.
Author: de Castilho, Aline Rogéria Freire
Duque, Cristiane
Negrini, Thaís de Cássia
Sacono, Nancy Tomoko
de Paula, Andréia Bolzan
de Souza Costa, Carlos Alberto
Spolidório, Denise Madalena Palomari
Puppin-Rontani, Regina Maria
Abstract: To evaluate: (1) the in vitro antibacterial, cytotoxic and mechanical properties of a resin-modified glass ionomer cement (RMGIC) containing different concentrations of chlorhexidine (CHX) and (2) the in vivo microbiologic action of the best concentration of CHX associated with the RMGIC applied on remaining dentine after indirect pulp treatment (IPT). For the in vitro studies, RMGIC was associated with 0.2, 0.5, 1.25 and 2.5% CHX. Microbiologic evaluation consisted of an agar diffusion test on cariogenic bacteria for 24h. Odontoblast-like cell metabolism and morphology analyses measured the cytotoxic effects of the RMGIC groups after 24h. The same groups were submitted to compressive and diametral tensile strength. The in vivo treatment consisted of IPT using an RMGIC associated with the best CHX concentration. Clinical and microbiologic evaluations were performed before and after 3 months. The use of 1.25% CHX significantly improved the antibacterial effects of the evaluated RMGIC, without causing any detrimental effects to the odontoblast-like cells and on the mechanical properties. This RMGIC and CHX combination completely eliminated mutans streptococci after 3 months of IPT. The RMGIC and 1.25% CHX mixture showed great biological and mechanical behaviour and could be a good treatment against caries progression. The association of CHX with a liner RMGIC opens a new perspective for arresting residual caries after IPT.
Subject: Actinomyces
Anti-infective Agents, Local
Cell Culture Techniques
Cell Line
Cell Shape
Cell Survival
Child, Preschool
Coloring Agents
Compressive Strength
Dental Caries
Dental Cavity Lining
Glass Ionomer Cements
Lactobacillus Acidophilus
Materials Testing
Mechanical Phenomena
Microbial Viability
Microscopy, Electron, Scanning
Resin Cements
Streptococcus Mutans
Stress, Mechanical
Tensile Strength
Tetrazolium Salts
Time Factors
Citation: Journal Of Dentistry. v. 41, n. 2, p. 155-63, 2013-Feb.
Rights: fechado
Identifier DOI: 10.1016/j.jdent.2012.10.014
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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