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Type: Artigo de periódico
Title: The Role Of Transient Receptor Potential A 1 (trpa1) In The Development And Maintenance Of Carrageenan-induced Hyperalgesia.
Author: Bonet, Ivan J M
Fischer, Luana
Parada, Carlos Amílcar
Tambeli, Claudia H
Abstract: Transient receptor potential ankyrin 1 (TRPA1) is a nonselective cation channel important in setting nociceptive threshold. It is expressed in nociceptive C-fibers and in non-neuronal cells involved in pro-inflammatory mediators' release. We asked whether TRPA1 contributes to carrageenan-induced hyperalgesia in rats, and if so, whether this contribution is mediated by mechanisms involved in inflammation such as cytokine release and neutrophil migration and/or by a direct sensitization of the primary afferent nociceptors. Pharmacological blockade of local TRPA1 by its selective antagonist HC 030031 prevented and reversed carrageenan-induced hyperalgesia, which was detected either by a mechanical or chemical (low dose of capsaicin) stimulus. However, it did not affect either carrageenan-induced cytokines expression or neutrophil migration. The neuronal TRPA1 gene silencing induced by intrathecal pre-treatment with antisense oligodoexynucleotide completely prevented carrageenan-induced hyperalgesia over 24 h and significantly reduced TRPA1 expression in the dorsal root ganglia cells (L5-6), which was not affected by carrageenan treatment. We conclude that TRPA1 plays an important role in the development and maintenance of carrageenan-induced inflammatory hyperalgesia by directly contributing to nociceptor excitability.
Subject: Acetanilides
Gene Silencing
Pain Measurement
Rats, Wistar
Trpc Cation Channels
Citation: Neuropharmacology. v. 65, p. 206-12, 2013-Feb.
Rights: fechado
Identifier DOI: 10.1016/j.neuropharm.2012.09.020
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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