Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200903
Type: Artigo de periódico
Title: Yeast Biomass Production: A New Approach In Glucose-limited Feeding Strategy.
Author: Vieira, Érika Durão
Andrietta, Maria da Graça Stupiello
Andrietta, Silvio Roberto
Abstract: The aim of this work was to implement experimentally a simple glucose-limited feeding strategy for yeast biomass production in a bubble column reactor based on a spreadsheet simulator suitable for industrial application. In biomass production process using Saccharomyces cerevisiae strains, one of the constraints is the strong tendency of these species to metabolize sugars anaerobically due to catabolite repression, leading to low values of biomass yield on substrate. The usual strategy to control this metabolic tendency is the use of a fed-batch process in which where the sugar source is fed incrementally and total sugar concentration in broth is maintained below a determined value. The simulator presented in this work was developed to control molasses feeding on the basis of a simple theoretical model in which has taken into account the nutritional growth needs of yeast cell and two input data: the theoretical specific growth rate and initial cell biomass. In experimental assay, a commercial baker's yeast strain and molasses as sugar source were used. Experimental results showed an overall biomass yield on substrate of 0.33, a biomass increase of 6.4 fold and a specific growth rate of 0.165 h(-1) in contrast to the predicted value of 0.180 h-1 in the second stage simulation.
Subject: Biomass
Bioreactors
Fermentation
Glucose
Molasses
Saccharomyces Cerevisiae
Saccharomyces Cerevisiae
Biomass
Fed-batch
Process Simulation
Yeast
Rights: aberto
Identifier DOI: 10.1590/S1517-83822013000200035
Address: http://www.ncbi.nlm.nih.gov/pubmed/24294254
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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