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Type: Artigo de periódico
Title: Comprehensive Two-dimensional Gas Chromatography Combined To Multivariate Data Analysis For Detection Of Disease-resistant Clones Of Eucalyptus.
Author: Hantao, Leandro Wang
Toledo, Bruna Regina
Ribeiro, Fabiana Alves de Lima
Pizetta, Marilia
Pierozzi, Caroline Geraldi
Furtado, Edson Luiz
Augusto, Fabio
Abstract: In this paper it is reported the use of the chromatographic profiles from volatile fractions of plant clones - in this case, hybrids of Eucalyptus grandis×Eucalyptus urophylla - to determine specimens susceptible to rust disease. The analytes were isolated by headspace solid phase microextraction (HS-SPME) and analyzed by comprehensive two-dimensional gas chromatography combined to fast quadrupole mass spectrometry (GC×GC-qMS). Parallel Factor Analysis (PARAFAC) was employed for estimate the correlation between the chromatographic profiles and resistance against Eucalyptus rust, after preliminary variable selection performed by Fisher ratio analysis. The proposed method allowed the differentiation between susceptible and non-susceptible clones and determination of three resistance biomarkers. This approach can be a valuable alternative for the otherwise time-consuming and labor-intensive methods commonly used.
Subject: Biological Markers
Gas Chromatography-mass Spectrometry
Multivariate Analysis
Plant Diseases
Plant Immunity
Plant Leaves
Solid Phase Microextraction
Volatile Organic Compounds
Comprehensive Two-dimensional Gas Chromatography
Mass Spectrometry
Metabolic Profile
Parallel Factor Analysis
Puccinia Psidii
Resistance Biomarkers
Citation: Talanta. v. 116, p. 1079-84, 2013-Nov.
Rights: fechado
Identifier DOI: 10.1016/j.talanta.2013.08.033
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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