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Type: Artigo de periódico
Title: Lignification In Sugarcane: Biochemical Characterization, Gene Discovery, And Expression Analysis In Two Genotypes Contrasting For Lignin Content.
Author: Bottcher, Alexandra
Cesarino, Igor
Santos, Adriana Brombini dos
Vicentini, Renato
Mayer, Juliana Lischka Sampaio
Vanholme, Ruben
Morreel, Kris
Goeminne, Geert
Moura, Jullyana Cristina Magalhães Silva
Nobile, Paula Macedo
Carmello-Guerreiro, Sandra Maria
Anjos, Ivan Antonio dos
Creste, Silvana
Boerjan, Wout
Landell, Marcos Guimarães de Andrade
Mazzafera, Paulo
Abstract: Sugarcane (Saccharum spp.) is currently one of the most efficient crops in the production of first-generation biofuels. However, the bagasse represents an additional abundant lignocellulosic resource that has the potential to increase the ethanol production per plant. To achieve a more efficient conversion of bagasse into ethanol, a better understanding of the main factors affecting biomass recalcitrance is needed. Because several studies have shown a negative effect of lignin on saccharification yield, the characterization of lignin biosynthesis, structure, and deposition in sugarcane is an important goal. Here, we present, to our knowledge, the first systematic study of lignin deposition during sugarcane stem development, using histological, biochemical, and transcriptional data derived from two sugarcane genotypes with contrasting lignin contents. Lignin amount and composition were determined in rind (outer) and pith (inner) tissues throughout stem development. In addition, the phenolic metabolome was analyzed by ultra-high-performance liquid chromatography-mass spectrometry, which allowed the identification of 35 compounds related to the phenylpropanoid pathway and monolignol biosynthesis. Furthermore, the Sugarcane EST Database was extensively surveyed to identify lignin biosynthetic gene homologs, and the expression of all identified genes during stem development was determined by quantitative reverse transcription-polymerase chain reaction. Our data provide, to our knowledge, the first in-depth characterization of lignin biosynthesis in sugarcane and form the baseline for the rational metabolic engineering of sugarcane feedstock for bioenergy purposes.
Subject: Bayes Theorem
Biosynthetic Pathways
Gene Expression Profiling
Gene Expression Regulation, Plant
Genes, Plant
Genetic Association Studies
Plant Stems
Principal Component Analysis
Citation: Plant Physiology. v. 163, n. 4, p. 1539-57, 2013-Dec.
Rights: fechado
Identifier DOI: 10.1104/pp.113.225250
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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