Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200853
Type: Artigo de periódico
Title: Increased Adhesive And Inflammatory Properties In Blood Outgrowth Endothelial Cells From Sickle Cell Anemia Patients.
Author: Sakamoto, Tatiana Mary
Lanaro, Carolina
Ozelo, Margareth Castro
Garrido, Vanessa Tonin
Olalla-Saad, Sara Teresinha
Conran, Nicola
Costa, Fernando Ferreira
Abstract: The endothelium plays an important role in sickle cell anemia (SCA) pathophysiology, interacting with red cells, leukocytes and platelets during the vaso-occlusive process and undergoing activation and dysfunction as a result of intravascular hemolysis and chronic inflammation. Blood outgrowth endothelial cells (BOECs) can be isolated from adult peripheral blood and have been used in diverse studies, since they have a high proliferative capacity and a stable phenotype during in vitro culture. This study aimed to establish BOEC cultures for use as an in vitro study model for endothelial function in sickle cell anemia. Once established, BOECs from steady-state SCA individuals (SCA BOECs) were characterized for their adhesive and inflammatory properties, in comparison to BOECs from healthy control individuals (CON BOECs). Cell adhesion assays demonstrated that control individual red cells adhered significantly more to SCA BOEC than to CON BOEC. Despite these increased adhesive properties, SCA BOECs did not demonstrate significant differences in their expression of major endothelial adhesion molecules, compared to CON BOECs. SCA BOECs were also found to be pro-inflammatory, producing a significantly higher quantity of the cytokine, IL-8, than CON BOECs. From the results obtained, we suggest that BOEC may be a good model for the in vitro study of SCA. Data indicate that endothelial cells of sickle cell anemia patients may have abnormal inflammatory and adhesive properties even outside of the chronic inflammatory and vaso-occlusive environment of patients.
Subject: Adolescent
Adult
Anemia, Sickle Cell
Case-control Studies
Cell Adhesion
Cell Adhesion Molecules
Cells, Cultured
Endothelial Cells
Erythrocytes
Female
Humans
Inflammation
Inflammation Mediators
Interleukin-8
Male
Middle Aged
Phenotype
Young Adult
Rights: fechado
Identifier DOI: 10.1016/j.mvr.2013.10.002
Address: http://www.ncbi.nlm.nih.gov/pubmed/24144783
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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