Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200836
Type: Artigo de periódico
Title: Epa Protects Against Muscle Damage In The Mdx Mouse Model Of Duchenne Muscular Dystrophy By Promoting A Shift From The M1 To M2 Macrophage Phenotype.
Author: Carvalho, Samara Camaçari de
Apolinário, Leticia Montanholi
Matheus, Selma Maria Michelin
Santo Neto, Humberto
Marques, Maria Julia
Abstract: In dystrophic mdx mice and in Duchenne muscular dystrophy, inflammation contributes to myonecrosis. Previously, we demonstrated that eicosapentaenoic acid (EPA) decreased inflammation and necrosis in dystrophic muscle. In the present study, we examined the effects of EPA and the corticoid deflazacort (DFZ) as modulators of M1 (iNOS-expressing cells) and M2 (CD206-expressing cells) macrophages. Mdx mice (14 days old) received EPA or DFZ for 16 days. The diaphragm, biceps brachii and quadriceps muscles were studied. Immunofluorescence, immunoblotting and ELISA assays showed that EPA increased interleucin-10, reduced interferon-γ and was more effective than DFZ in promoting a shift from M1 to M2.
Subject: Analysis Of Variance
Animals
Antigens, Differentiation
Creatine Kinase
Disease Models, Animal
Eicosapentaenoic Acid
Enzyme-linked Immunosorbent Assay
Female
Interferon-gamma
Interleukin-10
Lectins, C-type
Macrophages
Male
Mannose-binding Lectins
Mice
Mice, Inbred C57bl
Mice, Inbred Mdx
Muscles
Muscular Dystrophy, Duchenne
Nitric Oxide Synthase Type Ii
Phenotype
Pregnenediones
Receptors, Cell Surface
Deflazacort
Dystrophy
Epa
Inflammation
M1 Macrophages
M2 Macrophages
Rights: fechado
Identifier DOI: 10.1016/j.jneuroim.2013.09.007
Address: http://www.ncbi.nlm.nih.gov/pubmed/24090650
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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