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Type: Artigo de periódico
Title: Altered Red Cell And Platelet Adhesion In Hemolytic Diseases: Hereditary Spherocytosis, Paroxysmal Nocturnal Hemoglobinuria And Sickle Cell Disease.
Author: Sakamoto, Tatiana M
Canalli, Andreia A
Traina, Fabiola
Franco-Penteado, Carla F
Gambero, Sheley
Saad, Sara T O
Conran, Nicola
Costa, Fernando F
Abstract: Intravascular hemolysis may have important pathophysiological consequences, such as the induction of cellular adhesion and vasculopathy. We compared the adhesive properties of red cells (RBC) and platelets in hereditary spherocytosis (HS), paroxysmal nocturnal hemoglobinuria (PNH) and sickle cell disease (SCD) patients. The adhesion of RBC and platelets, from patients and healthy subjects, was determined using static adhesion assays. RBC surface markers were characterized by flow cytometry and lactate dehydrogenase (LDH), plasma hemoglobin (pHb) and TNF-α were assayed in serum/plasma samples. pHb levels were elevated in all three hemolytic diseases, indicating the incidence of intravascular hemolysis. RBC adhesion and TNF-α were augmented in HS and SCD, but not in PNH. Reticulocyte counts were raised in the three diseases, but were higher in HS and SCD than in PNH; high expressions of CD71, CD36 and CD49d were observed on SCD RBC, while CD71 alone was increased on HS and PNH RBC. Splenectomy was associated with reversals of increased pHb, RBC adhesion, reticulocytes, RBC marker expression and inflammation in HS. In contrast, platelet adhesion was elevated in SCD and PNH, but not HS. Platelet adhesion correlated significantly with serum LDH, but not pHb, in the hemolytic disease cohort; interestingly, LDH did not correlate with reticulocytes or pHb levels. Results indicate that extravascular, rather than intravascular, hemolysis (and ensuing RBC production) may contribute to elevations in RBC adhesive properties in HS and SCD, while mechanisms peculiar to each disease may augment platelet adhesion in SCD and PNH.
Subject: Adolescent
Anemia, Sickle Cell
Antigens, Cd
Antigens, Cd36
Blood Platelets
Case-control Studies
Hemoglobinuria, Paroxysmal
Integrin Alpha4
L-lactate Dehydrogenase
Middle Aged
Platelet Adhesiveness
Receptors, Transferrin
Reference Values
Reticulocyte Count
Spherocytosis, Hereditary
Tumor Necrosis Factor-alpha
Young Adult
Intravascular Hemolysis
Red Cells
Rights: fechado
Identifier DOI: 10.1016/j.clinbiochem.2013.09.011
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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