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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleNeuroprotection And Reduction Of Glial Reaction By Cannabidiol Treatment After Sciatic Nerve Transection In Neonatal Rats.pt_BR
dc.contributor.authorPerez, Matheuspt_BR
dc.contributor.authorBenitez, Suzana Upt_BR
dc.contributor.authorCartarozzi, Luciana Ppt_BR
dc.contributor.authorDel Bel, Elainept_BR
dc.contributor.authorGuimarães, Francisco Spt_BR
dc.contributor.authorOliveira, Alexandre L Rpt_BR
unicamp.authorMatheus Perez, Department of Structural and Functional Biology, Institute of Biology, University of Campinas (UNICAMP), CP 6109, CEP 13083-970, Campinas, SP, Brazil.pt_BR
unicamp.author.externalSuzana U Benitez,pt
unicamp.author.externalLuciana P Cartarozzi,pt
unicamp.author.externalElaine Del Bel,pt
unicamp.author.externalFrancisco S Guimarães,pt
unicamp.author.externalAlexandre L R Oliveira,pt
dc.subjectAnimalspt_BR
dc.subjectAnimals, Newbornpt_BR
dc.subjectCannabidiolpt_BR
dc.subjectCell Survivalpt_BR
dc.subjectNeurogliapt_BR
dc.subjectNeuroprotective Agentspt_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectSciatic Neuropathypt_BR
dc.subjectTreatment Outcomept_BR
dc.subjectApoptosispt_BR
dc.subjectCannabinoidpt_BR
dc.subjectDorsal Root Ganglia Neuronpt_BR
dc.subjectGlial Cellspt_BR
dc.subjectMotoneuronpt_BR
dc.description.abstractIn neonatal rats, the transection of a peripheral nerve leads to an intense retrograde degeneration of both motor and sensory neurons. Most of the axotomy-induced neuronal loss is a result of apoptotic processes. The clinical use of neurotrophic factors is difficult due to side effects and elevated costs, but other molecules might be effective and more easily obtained. Among them, some are derived from Cannabis sativa. Cannabidiol (CBD) is the major non-psychotropic component found on the surface of such plant leaves. The present study aimed to investigate the neuroprotective potential of CBD. Thus, 2-day-old Wistar rats were divided into the following experimental groups: sciatic nerve axotomy + CBD treatment (CBD group), axotomy + vehicle treatment (phosphate buffer group) and a control group (no-treatment group). The results were analysed by Nissl staining, immunohistochemistry and terminal deoxynucleotidyl transferase dUTP nick end labeling at 5 days post-lesion. Neuronal counting revealed both motor and sensory neuron rescue following treatment with CBD (15 and 30 mg/kg). Immunohistochemical analysis (obtained by synaptophysin staining) revealed 30% greater synaptic preservation within the spinal cord in the CBD-treated group. CBD administration decreased the astroglial and microglial reaction by 30 and 27%, respectively, as seen by glial fibrillary acidic protein and ionised calcium binding adaptor molecule 1 immunolabeling quantification. In line with such results, the terminal deoxynucleotidyl transferase dUTP nick end labeling reaction revealed a reduction of apoptotic cells, mostly located in the spinal cord intermediate zone, where interneurons promote sensory-motor integration. The present results show that CBD possesses neuroprotective characteristics that may, in turn, be promising for future clinical use.en
dc.relation.ispartofThe European Journal Of Neurosciencept_BR
dc.relation.ispartofabbreviationEur. J. Neurosci.pt_BR
dc.date.issued2013-Novpt_BR
dc.identifier.citationThe European Journal Of Neuroscience. v. 38, n. 10, p. 3424-34, 2013-Nov.pt_BR
dc.language.isoengpt_BR
dc.description.volume38pt_BR
dc.description.firstpage3424-34pt_BR
dc.rightsfechadopt_BR
dc.rights.holder© 2013 Federation of European Neuroscience Societies and John Wiley & Sons Ltd.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1460-9568pt_BR
dc.identifier.doi10.1111/ejn.12341pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/23981015pt_BR
dc.date.available2015-11-27T13:32:07Z-
dc.date.accessioned2015-11-27T13:32:07Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:32:07Z (GMT). No. of bitstreams: 1 pmed_23981015.pdf: 4242455 bytes, checksum: 972d6e11494cb6f89ec57593a5838458 (MD5) Previous issue date: 2013en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200793-
dc.identifier.idPubmed23981015pt_BR
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