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Type: Artigo de periódico
Title: Sildenafil (viagra) Protective Effects On Neuroinflammation: The Role Of Inos/no System In An Inflammatory Demyelination Model.
Author: Raposo, Catarina
Nunes, Ana Karolina de Santana
Luna, Rayana Leal de Almeida
Araújo, Shyrlene Meiry da Rocha
da Cruz-Höfling, Maria Alice
Peixoto, Christina Alves
Abstract: We recently demonstrated that sildenafil reduces the expression of cytokines, COX-2, and GFAP in a demyelinating model induced in wild-type (WT) mice. Herein, the understandings of the neuroprotective effect of sildenafil and the mediation of iNOS/NO system on inflammatory demyelination induced by cuprizone were investigated. The cerebella of iNOS(-/-) mice were examined after four weeks of treatment with cuprizone alone or combined with sildenafil. Cuprizone increased GFAP, Iba-1, TNF- α , COX-2, IL-1 β , and IFN- γ expression, decreased expression of glutathione S-transferase pi (GSTpi), and damaged myelin in iNOS(-/-) mice. Sildenafil reduced Iba-1, IFN- γ , and IL-1 β levels but had no effect on the expression of GFAP, TNF- α , and COX-2 compared to the cuprizone group. Sildenafil elevated GSTpi levels and improved the myelin structure/ultrastructure. iNOS(-/-) mice suffered from severe inflammation following treatment with cuprizone, while WT mice had milder inflammation, as found in the previous study. It is possible that inflammatory regulation through iNOS-feedback is absent in iNOS(-/-) mice, making them more susceptible to inflammation. Sildenafil has at least a partial anti-inflammatory effect through iNOS inhibition, as its effect on iNOS(-/-) mice was limited. Further studies are required to explain the underlying mechanism of the sildenafil effects.
Subject: Animals
Cyclic Gmp
Demyelinating Diseases
Glutathione S-transferase Pi
Mice, Knockout
Microscopy, Electron, Transmission
Microscopy, Fluorescence
Myelin Sheath
Nitric Oxide
Nitric Oxide Synthase Type Ii
Phosphodiesterase 5 Inhibitors
Rights: aberto
Identifier DOI: 10.1155/2013/321460
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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