Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200785
Type: Artigo de periódico
Title: Cafeteria Diet Inhibits Insulin Clearance By Reduced Insulin-degrading Enzyme Expression And Mrna Splicing.
Author: Brandimarti, P
Costa-Júnior, J M
Ferreira, S M
Protzek, A O
Santos, G J
Carneiro, E M
Boschero, A C
Rezende, L F
Abstract: Insulin clearance plays a major role in glucose homeostasis and insulin sensitivity in physiological and/or pathological conditions, such as obesity-induced type 2 diabetes as well as diet-induced obesity. The aim of the present work was to evaluate cafeteria diet-induced obesity-induced changes in insulin clearance and to explain the mechanisms underlying these possible changes. Female Swiss mice were fed either a standard chow diet (CTL) or a cafeteria diet (CAF) for 8 weeks, after which we performed glucose tolerance tests, insulin tolerance tests, insulin dynamics, and insulin clearance tests. We then isolated pancreatic islets for ex vivo glucose-stimulated insulin secretion as well as liver, gastrocnemius, visceral adipose tissue, and hypothalamus for subsequent protein analysis by western blot and determination of mRNA levels by real-time RT-PCR. The cafeteria diet induced insulin resistance, glucose intolerance, and increased insulin secretion and total insulin content. More importantly, mice that were fed a cafeteria diet demonstrated reduced insulin clearance and decay rate as well as reduced insulin-degrading enzyme (IDE) protein and mRNA levels in liver and skeletal muscle compared with the control animals. Furthermore, the cafeteria diet reduced IDE expression and alternative splicing in the liver and skeletal muscle of mice. In conclusion, a cafeteria diet impairs glucose homeostasis by reducing insulin sensitivity, but it also reduces insulin clearance by reducing IDE expression and alternative splicing in mouse liver; however, whether this mechanism contributes to the glucose intolerance or helps to ameliorate it remains unclear.
Subject: Animals
Dietary Carbohydrates
Dietary Fats
Disease Models, Animal
Eating
Female
Insulin
Insulin Resistance
Insulysin
Liver
Mice
Muscle, Skeletal
Obesity
Rna Splicing
Rna, Messenger
Cafeteria Diet
Diabetes
Insulin Clearance
Insulin-degrading Enzyme
Obesity
Rights: fechado
Identifier DOI: 10.1530/JOE-13-0177
Address: http://www.ncbi.nlm.nih.gov/pubmed/23959080
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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