Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200778
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleExtraction Of Tetracyclinic Antibiotic Residues From Fish Filet: Comparison And Optimization Of Different Procedures Using Liquid Chromatography With Fluorescence Detection.pt_BR
dc.contributor.authorOrlando, Eduardo Adilsonpt_BR
dc.contributor.authorSimionato, Ana Valéria Cpt_BR
unicamp.authorEduardo Adilson Orlando, Institute of Chemistry, University of Campinas - Unicamp, P.O. Box 6154, Campinas, SP, 13083-970, Brazil.pt_BR
unicamp.author.externalAna Valéria C Simionato,pt
dc.subjectAnimalspt_BR
dc.subjectAnti-bacterial Agentspt_BR
dc.subjectChromatography, High Pressure Liquidpt_BR
dc.subjectDrug Residuespt_BR
dc.subjectHydrogen-ion Concentrationpt_BR
dc.subjectLinear Modelspt_BR
dc.subjectLiquid-liquid Extractionpt_BR
dc.subjectReproducibility Of Resultspt_BR
dc.subjectSeafoodpt_BR
dc.subjectSensitivity And Specificitypt_BR
dc.subjectSolid Phase Extractionpt_BR
dc.subjectSpectrometry, Fluorescencept_BR
dc.subjectTetracyclinespt_BR
dc.subjectTilapiapt_BR
dc.subjectExtractionpt_BR
dc.subjectFactorial Experimental Designpt_BR
dc.subjectFishpt_BR
dc.subjectHplcpt_BR
dc.subjectResiduept_BR
dc.subjectTetracyclinespt_BR
dc.description.abstractAnti-microbials have been used to control the quality of the aquatic environment for both prophylactic and therapeutic purposes. Tetracyclines are among the main antimicrobials used in aquaculture, and present a particular difficulty for extraction, due to a complex structure and high interaction with components of the biological matrix. In this study, different techniques of extraction and clean-up of antimicrobials of the tetracycline class in tilapia filets have been optimized and compared, followed by validation of the methodology using the best procedure. Oxytetracycline, doxycycline, tetracycline and chlortetracycline were analyzed by HPLC-fluorescence under the following conditions: organic mobile phase composed of methanol:acetonitrile (1:1, v/v) and aqueous mobile phase containing sodium acetate (0.0375molL(-1)), calcium chloride (0.0175molL(-1)) and EDTA (0.0125molL(-1)) at pH 7.00. The chromatographic analysis was performed using a mobile phase gradient with a flow rate of 1mLmin(-1) and detection wavelength of 385/528nm (λexc/λem). Four extraction methods have been evaluated, namely: liquid-liquid partition; solid phase extraction (SPE) using phenyl, C18 and polymeric Oasis-HLB stationary phases; dispersive SPE (dSPE) using polymeric adsorbent XAD 16 resin; and QuEChERS. The methods have been optimized with fractional factorial experimental design and compared by the extraction efficiency. The liquid-liquid extraction and the QuEChERS methods showed low extraction efficiencies (14-30%) for the analytes. The use of dSPE showed good efficiency (40-60%), but with low precision and high consumption of time. Among the evaluated extraction techniques the use of SPE showed the best results, with emphasis on the phenyl phase (58-76%), and has been validated for analysis of residues of tetracyclines in tilapia muscle regarding selectivity, linearity, precision and limits of detection and quantification. The validated method was adequate for the investigation of the analytes at residue levels.en
dc.relation.ispartofJournal Of Chromatography. Apt_BR
dc.relation.ispartofabbreviationJ Chromatogr Apt_BR
dc.date.issued2013-Seppt_BR
dc.identifier.citationJournal Of Chromatography. A. v. 1307, p. 111-8, 2013-Sep.pt_BR
dc.language.isoengpt_BR
dc.description.volume1307pt_BR
dc.description.firstpage111-8pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2013 Elsevier B.V. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1873-3778pt_BR
dc.identifier.doi10.1016/j.chroma.2013.07.084pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/23932025pt_BR
dc.date.available2015-11-27T13:32:04Z-
dc.date.accessioned2015-11-27T13:32:04Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:32:04Z (GMT). No. of bitstreams: 1 pmed_23932025.pdf: 755205 bytes, checksum: 6be81a058f199f6d441f330dd399c145 (MD5) Previous issue date: 2013en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200778-
dc.identifier.idPubmed23932025pt_BR
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