Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200738
Type: Artigo de periódico
Title: Inhibition Of Human Platelet Aggregation By Eosinophils.
Author: Maziero, Aline Mendes
Lorenzetti, Raquel
Donato, José Luiz
Lilla, Sergio
De Nucci, Gilberto
Abstract: The relationship between the activity of eosinophils and platelets has been observed in recent decades by many scientists. These observations include increased numbers of eosinophils associated with platelet disorders, including changes in the coagulation cascade and platelet aggregation. Based on these observations, the interaction between eosinophils and platelets in platelet aggregation was analyze. Human platelets were incubated with eosinophil cytosolic fraction, promyelocytic human HL-60 clone 15 cell lineage, and eosinophil cationic protein (ECP). Platelet rich plasma (PRP) aggregation was induced by adenosine diphosphate, platelet activating factor, arachidonic acid, and collagen, and washed platelets (WP) were activated by thrombin. Aggregation induced by all agonists was dose dependently inhibited by eosinophil cytosolic fraction. This inhibition was only partially reversed by previous incubation of the eosinophils with l-Nitro-Arginine-Methyl-Ester (l-NAME). Previous incubation with indomethacin did not prevent the cytosolic fraction induced inhibition. The separation of eosinophil cytosolic fraction by gel filtration on Sephadex G-75 showed that the inhibitory activity was concentrated in the lower molecular weight fraction. HL-60 clone 15 cells differentiated into eosinophils for 5 and 7 day were able to inhibit platelet aggregation. The ECP protein inhibited the platelet aggregation on PRP and WP. This inhibition was more evident in WP, and the citotoxicity MTT assay proved the viability of tested platelets, showing that the observed inhibition by the ECP protein does not occur simply by cell death. Our results indicate that eosinophils play a fundamental role in platelet aggregation inhibition.
Subject: Adenosine Diphosphate
Animals
Arachidonic Acid
Blood Platelets
Chromatography, Gel
Collagen
Cytosol
Dextrans
Eosinophils
Hl-60 Cells
Humans
Indomethacin
Male
Molecular Weight
Ng-nitroarginine Methyl Ester
Platelet Activating Factor
Platelet Aggregation
Rats
Rats, Wistar
Thrombin
Time Factors
Eosinophil Cationic Protein
Eosinophil Cytosolic Fraction
Eosinophil Proteins
Platelet Aggregation Inhibition
Rights: fechado
Identifier DOI: 10.1016/j.lfs.2013.07.012
Address: http://www.ncbi.nlm.nih.gov/pubmed/23871985
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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