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Type: Artigo de periódico
Title: Potentiation Of High Hydrostatic Pressure Inactivation Of Mycobacterium By Combination With Physical And Chemical Conditions.
Author: de Souza, Ancelmo Rabelo
da Costa Demonte, Ana Luísa Silva Sampaio Moreira
de Araujo Costa, Karina
Faria, Mariana Alcantara Cardoso
Durães-Carvalho, Ricardo
Lancellotti, Marcelo
Bonafe, Carlos Francisco Sampaio
Abstract: Mycobacterium abscessus is an important hospital-acquired pathogen involved in infections associated with medical, surgical, and biopharmaceutical materials. In this work, we investigated the pressure-induced inactivation of two strains [2544 and American Type Culture Collection (ATCC) 19977] of M. abscessus in combination with different temperatures and pH conditions. For strain 2544, exposure to 250 MPa for 90 min did not significantly inactivate the bacteria at 20 °C, whereas at -15 °C, there was complete inactivation. Exposure to 250 MPa at ≥60 °C caused rapid inactivation, with no viable bacteria after 45 min. With 45 min of exposure, there were no viable bacteria at any temperature when a higher pressure (350 MPa) was used. Extremes of pH (4 or 9) also markedly enhanced the pressure-induced inactivation of bacteria at 250 MPa, with complete inactivation after 45 min. In comparison, exposure of this strain to the disinfecting agent glutaraldehyde (0.5 %) resulted in total inactivation within 5 min. Strain 19977 was more sensitive to high pressure but less sensitive to glutaraldehyde than strain 2544. These results indicate that high hydrostatic pressure in combination with other physical parameters may be useful in reducing the mycobacterial contamination of medical materials and pharmaceuticals that are sensitive to autoclaving.
Subject: Disinfection
Hydrogen-ion Concentration
Hydrostatic Pressure
Microbial Viability
Time Factors
Rights: fechado
Identifier DOI: 10.1007/s00253-013-5067-7
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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