Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200715
Type: Artigo de periódico
Title: Optimal Single-embryo Mass Spectrometry Fingerprinting.
Author: Tata, Alessandra
Sudano, Mateus J
Santos, Vanessa G
Landim-Alvarenga, Fernanda D C
Ferreira, Christina R
Eberlin, Marcos N
Abstract: In pre-implantation embryos, lipids play key roles in determining viability, cryopreservation and implantation properties, but often their analysis is analytically challenging because of the few picograms of analytes present in each of them. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) allows obtaining individual phospholipid profiles of these microscopic organisms. This technique is sensitive enough to enable analysis of individual intact embryos and monitoring the changes in membrane lipid composition in the early stages of development serving as screening method for studies of biology and biotechnologies of reproduction. This article introduces an improved, more comprehensive MALDI-MS lipid fingerprinting approach that considerably increases the lipid information obtained from a single embryo. Using bovine embryos as a biological model, we have also tested optimal sample storage and handling conditions before the MALDI-MS analysis. Improved information at the molecular level is provided by the use of a binary matrix that enables phosphatidylcholines, sphingomyelins, phosphatidylserines, phosphatidylinositols and phosphoethanolamines to be detected via MALDI(±)-MS in both the positive and negative ion modes. An optimal MALDI-MS protocol for lipidomic monitoring of a single intact embryo is therefore reported with potential applications in human and animal reproduction, cell development and stem cell research.
Subject: Animals
Blastocyst
Cattle
Female
Male
Membrane Lipids
Phospholipids
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Binary Matrix
Embryo
Fingerprinting
Matrix-assisted Laser Desorption-mass Spectrometry (maldi-ms)
Phospholipids
Rights: fechado
Identifier DOI: 10.1002/jms.3231
Address: http://www.ncbi.nlm.nih.gov/pubmed/23832940
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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