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Type: Artigo de periódico
Title: Ion Mobility Spectrometry Focusing On Speciation Analysis Of Metals/metalloids Bound To Carbonic Anhydrase.
Author: Pessôa, Gustavo de Souza
Pilau, Eduardo Jorge
Gozzo, Fábio Cesar
Arruda, Marco Aurélio Zezzi
Abstract: In the present work, traveling wave ion mobility spectrometry-mass spectrometry (TWIMS-MS) was applied to speciation analysis of metalloproteins. The influence of pH on complexation conditions between some metals and bovine carbonic anhydrase was evaluated from pH 6 to 9, as well as the time involved in their complexation (0-24 h). Employing TWIMS-MS, two conformational states of bovine carbonic anhydrase were observed with charge states of +12 and +11; these configurations being evaluated in terms of the folded state of the apo form and this protein (at charge state +11) being linked to barium, lead, copper, and zinc in their divalent forms. Metalloprotein speciation analysis was carried out for copper (Cu(+) and Cu(2+)), lead (Pb(2+) and Pb(4+)), and selenium (Se(4+) and Se(6+)) species complexed with bovine carbonic anhydrase. Mobilities of all complexed species were compared, also considering the apo form of this protein.
Subject: Carbonic Anhydrases
Hydrogen-ion Concentration
Ion Transport
Protein Binding
Spectrometry, Mass, Electrospray Ionization
Citation: Analytical And Bioanalytical Chemistry. v. 405, n. 24, p. 7653-60, 2013-Sep.
Rights: fechado
Identifier DOI: 10.1007/s00216-013-7064-1
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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