Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200633
Type: Artigo de periódico
Title: Modulatory Effects Of Yerba Maté (ilex Paraguariensis) On The Pi3k-akt Signaling Pathway.
Author: Arçari, Demétrius Paiva
Santos, Juliana Carvalho
Gambero, Alessandra
Ferraz, Lucio Fábio Caldas
Ribeiro, Marcelo Lima
Abstract: The aim of this study was to evaluate the effects of yerba maté (YM) extract on the phosphatidylinositol 3-kinase (PI3K)-AKT signaling pathway in vivo. The mice were introduced to either standard- or high-fat diet (HFD). After 8 weeks on an HFD, mice were randomly assigned to one of the two treatment conditions, water or yerba maté extract at 1.0 g/kg. After treatment, glucose blood level and hepatic insulin response were evaluated. Liver tissue was examined to determine the mRNA levels using the PI3K-AKT PCR array. The nuclear translocation of forkhead box O1 (FOXO1) was determined by an electrophoretic mobility-shift assay. Our data demonstrated that yerba maté extract significantly decreased the final body weight, glucose blood levels, and insulin resistance of mice. Molecular analysis demonstrated that an HFD downregulated Akt2, Irs1, Irs2, Pi3kca, Pi3kcg, and Pdk1; after yerba maté treatment, the levels of those genes returned to baseline. In addition, an HFD upregulated Pepck and G6pc and increased FOXO1 nuclear translocation. The intervention downregulated these genes by decreasing FOXO1 nuclear translocation. The results obtained demonstrate for the first time the specific action of yerba maté on the PI3K-AKT pathway, which contributed to the observed improvement in hepatic insulin signaling.
Subject: Animals
Blood Glucose
Body Weight
Diet, High-fat
Down-regulation
Forkhead Transcription Factors
Glucose-6-phosphatase
Ilex Paraguariensis
Insulin
Insulin Receptor Substrate Proteins
Insulin Resistance
Male
Mice
Phosphatidylinositol 3-kinase
Protein-serine-threonine Kinases
Proto-oncogene Proteins C-akt
Rna, Messenger
Signal Transduction
Up-regulation
Insulin Resistance
Obesity
Pi3k-akt
Yerba Maté
Rights: fechado
Identifier DOI: 10.1002/mnfr.201200834
Address: http://www.ncbi.nlm.nih.gov/pubmed/23670797
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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