Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200586
Type: Artigo de periódico
Title: Molecular Matching Of Red Blood Cells Is Superior To Serological Matching In Sickle Cell Disease Patients.
Author: da Costa, Daiane Cobianchi
Pellegrino, Jordão
Guelsin, Gláucia Andréia Soares
Ribeiro, Karina Antero Rosa
Gilli, Simone Cristina Olenscki
Castilho, Lilian
Abstract: To evaluate the usefulness of DNA methods to provide a means to precisely genotypically match donor blood units for the antigen-negative type of 35 sickle cell disease patients. Red blood cell units were investigated for ABO, D, C, c, E, e, K, Fy(a), Fy(b), Jk(a), Jk(b), S, s, Di(a) and RH variants by performing a molecular array (Human Erythrocyte Antigen BeadChip(TM), BioArray Solutions), polymerase chain reaction followed by restriction fragment length polymorphism analysis and sequencing of patient samples and donor units that had been serologically matched based on the ABO, Rh and K phenotypes and the presence of antibodies. Matches for 21 of 35 sickle cell disease patients presented discrepancies or mismatches for multiple antigens between the genotype profile and the antigen profile of their serologically-matched blood units. The main discrepancies or mismatches occurred in the RH, FY, JK and MNS systems. Eight Rh alloimmunized patients presented RHD and RHCE variants that had not been serologically identified. According to these results better matches were found for the patients with genotyped units and the patients benefited as shown by better in vivo red blood cell survival. Molecular matching is superior to serological matching in sickle cell disease patients, decreasing the risk of transfusion reactions, especially delayed transfusion reactions to existing alloantibodies and preventing alloimmunization.
Subject: Anemia, Sickle Cell
Blood Group Antigens
Isoantibodies/blood
Molecular Typing
Rights: aberto
Identifier DOI: 10.5581/1516-8484.20130012
Address: http://www.ncbi.nlm.nih.gov/pubmed/23580882
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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