Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200532
Type: Artigo de periódico
Title: Biochemical Characterization And Pharmacological Properties Of New Basic Pla2 Brtx-i Isolated From Bothrops Roedingeri (roedinger's Lancehead) Mertens, 1942, Snake Venom.
Author: Gomes Heleno, Mauricio Aurelio
Baldasso, Paulo Aparecido
Ponce-Soto, Luis Alberto
Marangoni, Sérgio
Abstract: BrTX-I, a PLA2, was purified from Bothrops roedingeri venom after only one chromatographic step using reverse-phase HPLC on μ-Bondapak C-18 column. A molecular mass of 14358.69 Da was determined by MALDI-TOF mass spectrometry. Amino acid analysis showed a high content of hydrophobic and basic amino acids as well as 14 half-cysteine residues. The total amino acid sequence was obtained using SwissProt database and showed high amino acid sequence identity with other PLA2 from snake venom. The amino acid composition showed that BrTX-I has a high content of Lys, Tyr, Gly, Pro, and 14 half-Cys residues, typical of a basic PLA2. BrTX-I presented PLA2 activity and showed a minimum sigmoidal behavior, reaching its maximal activity at pH 8.0, 35-45°C, and required Ca(2+). In vitro, the whole venom and BrTX-I caused a neuromuscular blockade in biventer cervicis preparations in a similar way to other Bothrops species. BrTX-I induced myonecrosis and oedema-forming activity analyzed through injection of the purified BrTX-I in mice. Since BrTX-I exerts a strong proinflammatory effect, the enzymatic phospholipid hydrolysis might be relevant for these phenomena; incrementing levels of IL-1, IL-6, and TNF α were observed at 15 min, 30 min, one, two, and six hours postinjection, respectively.
Subject: Amino Acids
Animals
Bothrops
Chickens
Chromatography, High Pressure Liquid
Cysteine
Cytokines
Edema
Hydrogen-ion Concentration
Hydrolysis
Inflammation
Male
Mice
Peptides
Phospholipases A2
Phospholipases A2, Secretory
Reptilian Proteins
Snake Venoms
Spectrometry, Mass, Electrospray Ionization
Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization
Temperature
Rights: aberto
Identifier DOI: 10.1155/2013/591470
Address: http://www.ncbi.nlm.nih.gov/pubmed/23509747
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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