Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200521
Type: Artigo de periódico
Title: Detection Of Tcd4+ Subsets In Human Carotid Atheroma.
Author: Oliveira, Rômulo Tadeu Dias
Silva, Rosiane Maria
Teo, Fabio Haach
Mineiro, Marcela Franco
Ferreira, Maria Carolina
Altemani, Albina
Mamoni, Ronei Luciano
Menezes, Fábio Husseman
Blotta, Maria Heloisa Souza Lima
Abstract: Activated TCD4(+) cells are detected in human atherosclerotic plaques which indicate their participation in disease progression and destabilization. Among these cells, IFN-γ-producing T cells (TH1) are recognized as having a pro-atherogenic role. Recently, the IL-17-producing T helper lineage of cells (TH17) has been identified in atherosclerotic lesions. They have been linked to atheroma development through the production of pro-inflammatory mediators present in these lesions. Furthermore, IL-22 producing TCD4(+) cells (TH22) have been identified in the atheromatous environment, but their presence and function has not been investigated. The aim of this study was to analyze the immune response mediated by pro-inflammatory subtypes of TCD4(+) cells in atheromatous lesions. Atherosclerotic plaques of 57 patients with critical stenosis of carotid submitted to endarterectomy were evaluated. Three carotid fragments from organ donors were used as control. mRNA analysis showed expression of TH1 (IFN-γ, T-bet, IL-2, IL-12p35, TNF-α and IL-18); TH2 (GATA-3); TH17 (IL-17A, IL-17RA, Rorγt, TGF-β, IL-6, IL-1β, IL-23p19, CCL20, CCR4 and CCR6) and TH22 (IL-22 and Ahr) related markers. Asymptomatic patients showed higher expression of mRNA of IL-10, TGF-β, CCR4 and GATA-3 when compared to symptomatic ones. Immunohistochemistry analysis showed higher levels of IL-23, TGF-β, IL-1β and IL-18 in macrophages and foam cells in unstable lesions compared to stable and control ones. In vitro stimulation of atheroma cells induced IL-17 and IFN-γ production. Finally we were able to detect, the following subpopulations of TCD3(+) cells: TCD4(+) IFN-γ(+), TCD4(+)IL-17(+), TCD4(+)IL-4(+), TCD4(+)IL-22(+) and double positive cells (IFN-γ/IL-17(+), IFN-γ/IL-22(+) or IL-17/IL-22(+)). Our results showed the presence of distinct TCD4(+) cells subsets in human carotid lesions and suggest that interactions among them may contribute to the atheroma progression and destabilization.
Subject: Adult
Aged
Aged, 80 And Over
Cd4-positive T-lymphocytes
Carotid Arteries
Endarterectomy
Female
Gata3 Transcription Factor
Humans
Immunohistochemistry
Interleukin-10
Lymphocyte Subsets
Male
Middle Aged
Plaque, Atherosclerotic
Rna, Messenger
Receptors, Ccr4
Transforming Growth Factor Beta
Rights: fechado
Identifier DOI: 10.1016/j.cyto.2013.02.004
Address: http://www.ncbi.nlm.nih.gov/pubmed/23474106
Date Issue: 2013
Appears in Collections:Unicamp - Artigos e Outros Documentos

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