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Type: Artigo de periódico
Title: Temporal Relationship Between Aquaporin-4 And Glial Fibrillary Acidic Protein In Cerebellum Of Neonate And Adult Rats Administered A Bbb Disrupting Spider Venom.
Author: Stavale, Leila Miguel
Soares, Edilene Siqueira
Mendonça, Monique C P
Irazusta, Silvia P
da Cruz Höfling, Maria Alice
Abstract: Two astrocyte markers, the glial water channel aquaporin-4 (AQP4) and the glial fibrillary acidic protein (GFAP), have been implicated in several physiological and pathological conditions in the central nervous system (CNS) as well as in blood-brain barrier breakdown (BBBb). By color segmentation the immunoreactivity of both proteins, we demonstrate that the expression of AQP4 and GFAP was increased in the cerebellum of neonate (14-day-old, P14) and adult (8-week-old) rats administered Phoneutria nigriventer spider venom (PNV) known to cause perivascular edema, BBBb and convulsion. In the cerebellum's gray matter, PNV produced a major response, especially in the granular layer. Parallel increases in AQP4 and GFAP expression occurred 24 h after envenomation in the white matter of P14 and in the molecular layer of adults, as well as in the granular layer 2 h after envenomation. In the Purkinje layer there was a tendency of increased AQP4, for both, neonates (5 h), and adults (2 and 24 h). Moreover, PNV also provoked nonparallel upregulation of both markers with prevalence of upregulation of AQP 4 for P14 rats, and GFAP for adults. The major expression of both proteins was in the gray matter. The data indicates a venom effect in water/electrolyte balance in the cerebellum and the participation of AQP4 in these effects. Age-related and time-related regional differences probably reflect specificity in AQP4 distribution in different astrocytic membrane domains as well as its participation in K(+) buffering and neural activity. This study is the first to associate astrocytic AQP4 expression and reactive gliosis in a model of BBB permeability promoted by P. nigriventer venom. Our data provide compelling evidence that AQP4 expression was increased in the cerebellum of rats administered PNV.
Subject: Age Factors
Animals, Newborn
Aquaporin 4
Biological Markers
Blood-brain Barrier
Cell Membrane Permeability
Glial Fibrillary Acidic Protein
Image Processing, Computer-assisted
Rats, Wistar
Spider Venoms
Time Factors
Rights: fechado
Identifier DOI: 10.1016/j.toxicon.2013.01.026
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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