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dc.typeArtigo de periódicopt_BR
dc.titlePcr-based Detection And Genotyping Of Helicobacter Pylori In Endoscopic Biopsy Samples From Brazilian Patients.pt_BR
dc.contributor.authorMenoni, Silvia M Ferreirapt_BR
dc.contributor.authorBonon, Sandra Helena Alvespt_BR
dc.contributor.authorZeitune, José Murilo Robilotapt_BR
dc.contributor.authorCosta, Sandra Cecília Botelhopt_BR
unicamp.authorSilvia M Ferreira Menoni, Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13081-970 Campinas, SP, Brazilpt_BR Course, Federal University of Mato Grosso do Sul (UFMS), 79620-080 Três Lagoas, MS, Helena Alves Bonon,pté Murilo Robilota Zeitune,pt Cecília Botelho Costa,pt
dc.description.abstractHelicobacter pylori (H. pylori) is considered the second most prevalent infection in man. A precise diagnosis is important for treating patients with the indicative gastrointestinal symptoms. The present study analyzes the effectiveness of a molecular biology method (PCR) comparing the results obtained with the histology and with the rapid urease tests. PCR was used in the detection and genotyping of the H. pylori urease-C gene and the patterns which were obtained from the patients studied. 141 biopsy samples from 131 patients were evaluated. 59 paraffin biopsies samples were positive for H. pylori according to the histological examination. Of those, 59/12 (20.3%) were amplified using PCR. Of the 82 samples from the fresh biopsies, 64 were positive for H. pylori according to the rapid urease test (78%); there was an agreement of 100% with PCR. Sixty positive H. pylori samples were genotyped (58 samples of fresh biopsies and 2 samples of paraffin biopsies) using two restriction enzymes. The patterns observed were analyzed with the computational program BIO 1D; 11 patterns with the enzyme HhaI and 12 patterns with the enzyme MboI were found. However, it was not possible to find a statistically significant correlation between the specific genotypes and digestive pathologies. Accordingly, future research should be performed to confirm a statistically significant relationship between genotyping and gastrointestinal symptoms.en
dc.relation.ispartofGastroenterology Research And Practicept_BR
dc.relation.ispartofabbreviationGastroenterol Res Practpt_BR
dc.identifier.citationGastroenterology Research And Practice. v. 2013, p. 951034, 2013.pt_BR
dc.description.provenanceMade available in DSpace on 2015-11-27T13:31:21Z (GMT). No. of bitstreams: 1 pmed_23401678.pdf: 4116858 bytes, checksum: efc505aac33c059beb64adb2eb29ec65 (MD5) Previous issue date: 2013en
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