Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200431
Type: Artigo de periódico
Title: Establishing The Feasibility Of Using β-glucosidase Entrapped In Lentikats And In Sol-gel Supports For Cellobiose Hydrolysis.
Author: Figueira, Joelise A
Sato, Hélia H
Fernandes, Pedro
Abstract: β-Glucosidases represent an important group of enzymes due to their pivotal role in various biotechnological processes. One of the most prominent is biomass degradation for the production of fuel ethanol from cellulosic agricultural residues and wastes, where the use of immobilized biocatalysts may prove advantageous. Within such scope, the present work aimed to evaluate the feasibility of entrapping β-glucosidase in either sol-gel or in Lentikats supports for application in cellobiose hydrolysis, and to perform the characterization of the resulting bioconversion systems. The activity and stability of the immobilized biocatalyst over given ranges of temperature and pH values were assessed, as well as kinetic data, and compared to the free form, and the operational stability was evaluated. Immobilization increased the thermal stability of the enzyme, with a 10 °C shift to an optimal temperature in the case of sol-gel support. Mass transfer hindrances as a result of immobilization were not significant, for sol-gel support. Lentikats-entrapped glucosidase was used in 19 consecutive batch runs for cellobiose hydrolysis, without noticeable decrease in product yield. Moreover, encouraging results were obtained for continuous operation. In the overall, the feasibility of using immobilized biocatalysts for cellobiose hydrolysis was established.
Subject: Biomass
Cellobiose
Enzymes, Immobilized
Feasibility Studies
Gels
Hydrogen-ion Concentration
Hydrolysis
Microscopy, Electron, Scanning
Polymethyl Methacrylate
Temperature
Beta-glucosidase
Rights: fechado
Identifier DOI: 10.1021/jf304594s
Address: http://www.ncbi.nlm.nih.gov/pubmed/23294439
Date Issue: 2013
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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