Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200408
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dc.contributor.CRUESPUNIVERSIDADE ESTADUAL DE CAMPINASpt_BR
dc.contributor.authorunicampAlvarenga, Elenice Montept_BR
dc.contributor.authorunicampVidal, Benedicto de Campospt_BR
dc.contributor.authorunicampMello, Maria Luiza Silveirapt_BR
dc.typeArtigopt_BR
dc.titleContribution of AT-, GC-, and methylated cytidine-rich DNA to chromatin composition in Malpighian tubule cell nuclei of Panstrongylus megistus (hemiptera, Reduviidae)pt_BR
dc.contributor.authorAlvarenga, Elenice M.pt_BR
dc.contributor.authorMondin, Mateuspt_BR
dc.contributor.authorRodrigues, Vera L.C.C.pt_BR
dc.contributor.authorAndrade, Larissa M.pt_BR
dc.contributor.authorVidal, Benedicto de Campospt_BR
dc.contributor.authorMello, Maria Luiza S.pt_BR
dc.subjectPanstrongylus megistuspt_BR
dc.subjectHeterocromatinapt_BR
dc.subjectDNApt_BR
dc.subjectEpigenéticapt_BR
dc.subjectMicroscopia de fluorescênciapt_BR
dc.subjectTúbulos de Malpighipt_BR
dc.subject.otherlanguagePanstrongylus megistuspt_BR
dc.subject.otherlanguageHeterochromatinpt_BR
dc.subject.otherlanguageDNApt_BR
dc.subject.otherlanguageEpigeneticspt_BR
dc.subject.otherlanguageMicroscopy, Fluorescencept_BR
dc.subject.otherlanguageMalpighian tubulespt_BR
dc.description.abstractThe Malpighian tubule cell nuclei of male Panstrongylus megistus, a vector of Chagas disease, contain one chromocenter, which is composed solely of the Y chromosome. Considering that different chromosomes contribute to the composition of chromocenters in different triatomini species, the aim of this study was to determine the contribution of AT-, GC-, and methylated cytidine-rich DNA in the chromocenter as well as in euchromatin of Malpighian tubule cell nuclei of P. megistus in comparison with published data for Triatoma infestans. Staining with 4′,6-diamidino-2-phenylindole/actinomycin D and chromomycin A3/distamycin, immunodetection of 5-methylcytidine and AgNOR test were used. The results revealed AT-rich/GC-poor DNA in the male chromocenter, but equally distributed AT and GC DNA sequences in male and female euchromatin, like in T. infestans. Accumulation of argyrophilic proteins encircling the chromocenter did not always correlate with that of GC-rich DNA. Methylated DNA identified by immunodetection was found sparsely distributed in the euchromatin of both sexes and at some points around the chromocenter edge, but it could not be considered responsible for chromatin condensation in the chromocenter, like in T. infestans. However, unlike in T. infestans, no correlation between the chromocenter AT-rich DNA and nucleolus organizing region (NOR) DNA was found in P. megistus.pt_BR
dc.relation.ispartofActa histochemicapt_BR
dc.relation.ispartofabbreviationActa histochem.pt_BR
dc.publisher.cityMuenchenpt_BR
dc.publisher.countryAlemanhapt_BR
dc.publisherElsevierpt_BR
dc.date.issued2012pt_BR
dc.date.monthofcirculationNov.pt_BR
dc.language.isoengpt_BR
dc.description.volume114pt_BR
dc.description.issuenumber7pt_BR
dc.description.firstpage665pt_BR
dc.description.lastpage672pt_BR
dc.rightsfechadopt_BR
dc.sourceWOSpt_BR
dc.identifier.issn0065-1281pt_BR
dc.identifier.eissn1618-0372pt_BR
dc.identifier.doi10.1016/j.acthis.2011.12.001pt_BR
dc.identifier.urlhttps://www.sciencedirect.com/science/article/pii/S006512811100184Xpt_BR
dc.description.sponsorshipCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOpt_BR
dc.description.sponsorshipFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOpt_BR
dc.description.sponsordocumentnumber47303/2009-7; 132341/2010-7; 301943/2009-5pt_BR
dc.description.sponsordocumentnumber2010/50015-6pt_BR
dc.date.available2015-11-27T13:29:17Z-
dc.date.accessioned2015-11-27T13:29:17Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:29:17Z (GMT). No. of bitstreams: 0 Previous issue date: 2012. Added 1 bitstream(s) on 2019-03-14T14:32:34Z : No. of bitstreams: 1 22197484.pdf: 1059443 bytes, checksum: 7fac9184bca34bec0e67197612cd2263 (MD5) Bitstreams deleted on 2020-06-15T14:11:44Z: 22197484.pdf,. Added 1 bitstream(s) on 2021-01-04T15:13:48Z : No. of bitstreams: 1 000307864700005.pdf: 1123859 bytes, checksum: 1fb2ed88a6b3c97b6b330867f81f629a (MD5)en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200408-
dc.contributor.departmentsem informaçãopt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.departmentDepartamento de Biologia Estrutural e Funcionalpt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.contributor.unidadeInstituto de Biologiapt_BR
dc.subject.keywordP. megistuspt_BR
dc.subject.keywordAgNORpt_BR
dc.identifier.source000307864700005-
dc.creator.orcidsem informaçãopt_BR
dc.creator.orcid0000-0003-0285-1653pt_BR
dc.creator.orcid0000-0003-1629-5619pt_BR
dc.type.formArtigopt_BR
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