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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleEndurance Training Stimulates Growth And Survival Pathways And The Redox Balance In Rat Pancreatic Islets.pt_BR
dc.contributor.authorCalegari, Vivian Cpt_BR
dc.contributor.authorAbrantes, Julia Lpt_BR
dc.contributor.authorSilveira, Leonardo Rpt_BR
dc.contributor.authorPaula, Flavia Mpt_BR
dc.contributor.authorCosta, José Mariapt_BR
dc.contributor.authorRafacho, Alexpt_BR
dc.contributor.authorVelloso, Lício Apt_BR
dc.contributor.authorCarneiro, Everardo Mpt_BR
dc.contributor.authorBosqueiro, Jose Rpt_BR
dc.contributor.authorBoschero, Antonio Cpt_BR
dc.contributor.authorZoppi, Claudio Cpt_BR
unicamp.authorVivian C Calegari, Dept. of Anatomy, Cellular Biology and Biophysics and Physiology, Institute of Biology, State Univ. of Campinas (UNICAMP). P.O. Box 6109, Campinas, SP. CEP: 13083-865, Brazil.pt_BR
unicamp.author.externalJulia L Abrantes,pt
unicamp.author.externalLeonardo R Silveira,pt
unicamp.author.externalFlavia M Paula,pt
unicamp.author.externalJosé Maria Costa,pt
unicamp.author.externalAlex Rafacho,pt
unicamp.author.externalLício A Velloso,pt
unicamp.author.externalEverardo M Carneiro,pt
unicamp.author.externalJose R Bosqueiro,pt
unicamp.author.externalAntonio C Boschero,pt
unicamp.author.externalClaudio C Zoppi,pt
dc.subjectAnimalspt_BR
dc.subjectAntioxidantspt_BR
dc.subjectApoptosispt_BR
dc.subjectBody Weightpt_BR
dc.subjectFatiguept_BR
dc.subjectGene Expressionpt_BR
dc.subjectGlucosept_BR
dc.subjectInsulinpt_BR
dc.subjectInsulin-secreting Cellspt_BR
dc.subjectIslets Of Langerhanspt_BR
dc.subjectMalept_BR
dc.subjectOxidation-reductionpt_BR
dc.subjectPhosphorylationpt_BR
dc.subjectPhysical Conditioning, Animalpt_BR
dc.subjectPhysical Endurancept_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.subjectReactive Oxygen Speciespt_BR
dc.subjectSignal Transductionpt_BR
dc.description.abstractEndurance training has been shown to increase pancreatic β-cell function and mass. However, whether exercise modulates β-cell growth and survival pathways signaling is not completely understood. This study investigated the effects of exercise on growth and apoptotic markers levels in rat pancreatic islets. Male Wistar rats were randomly assigned to 8-wk endurance training or to a sedentary control group. After that, pancreatic islets were isolated; gene expression and the total content and phosphorylation of several proteins related to growth and apoptotic pathways as well as the main antioxidant enzymes were determined by real-time polymerase chain reaction and Western blot analysis, respectively. Reactive oxygen species (ROS) production was measured by fluorescence. Endurance training increased the time to reach fatigue by 50%. Endurance training resulted in increased protein phosphorylation content of AKT (75%), AKT substrate (AS160; 100%), mTOR (60%), p70s6k (90%), and ERK1/2 (50%), compared with islets from control group. Catalase protein content was 50% higher, whereas ROS production was 49 and 77% lower in islets from trained rats under basal and stimulating glucose conditions, respectively. Bcl-2 mRNA and protein levels increased by 46 and 100%, respectively. Bax and cleaved caspase-3 protein contents were reduced by 25 and 50% in islets from trained rats, respectively. In conclusion, these results demonstrate that endurance training favors the β-cell growth and survival by activating AKT and ERK1/2 pathways, enhancing antioxidant capacity, and reducing ROS production and apoptotic proteins content.en
dc.relation.ispartofJournal Of Applied Physiology (bethesda, Md. : 1985)pt_BR
dc.relation.ispartofabbreviationJ. Appl. Physiol.pt_BR
dc.date.issued2012-Marpt_BR
dc.identifier.citationJournal Of Applied Physiology (bethesda, Md. : 1985). v. 112, n. 5, p. 711-8, 2012-Mar.pt_BR
dc.language.isoengpt_BR
dc.description.volume112pt_BR
dc.description.firstpage711-8pt_BR
dc.rightsfechadopt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1522-1601pt_BR
dc.identifier.doi10.1152/japplphysiol.00318.2011pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/22174407pt_BR
dc.date.available2015-11-27T13:29:16Z-
dc.date.accessioned2015-11-27T13:29:16Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:29:16Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200399-
dc.identifier.idPubmed22174407pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

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