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Type: Artigo de periódico
Title: Drosophila Melanogaster S2 Cells For Expression Of Heterologous Genes: From Gene Cloning To Bioprocess Development.
Author: Moraes, Angela M
Jorge, Soraia A C
Astray, Renato M
Suazo, Claudio A T
Calderón Riquelme, Camilo E
Augusto, Elisabeth F P
Tonso, Aldo
Pamboukian, Marilena M
Piccoli, Rosane A M
Barral, Manuel F
Pereira, Carlos A
Abstract: In the present review we discuss strategies that have been used for heterologous gene expression in Drosophila melanogaster Schneider 2 (S2) cells using plasmid vectors. Since the growth of S2 cells is not dependent on anchorage to solid substrates, these cells can be easily cultured in suspension in large volumes. The factors that most affect the growth and gene expression of S2 cells, namely cell line, cell passage, inoculum concentration, culture medium, temperature, dissolved oxygen concentration, pH, hydrodynamic forces and toxic metabolites, are discussed by comparison with other insect and mammalian cells. Gene expression, cell metabolism, culture medium formulation and parameters involved in cellular respiration are particularly emphasized. The experience of the authors with the successful expression of a biologically functional protein, the rabies virus glycoprotein (RVGP), by recombinant S2 cells is presented in the topics covered.
Subject: Animals
Animals, Genetically Modified
Cell Culture Techniques
Cell Line
Culture Media
Drosophila Melanogaster
Gene Expression
Rabies Virus
Viral Envelope Proteins
Rights: fechado
Identifier DOI: 10.1016/j.biotechadv.2011.10.009
Date Issue: -1-Uns- -1
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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