Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200344
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleSafranine As A Fluorescent Probe For The Evaluation Of Mitochondrial Membrane Potential In Isolated Organelles And Permeabilized Cells.pt_BR
dc.contributor.authorFigueira, Tiago Rpt_BR
dc.contributor.authorMelo, Daniela Rpt_BR
dc.contributor.authorVercesi, Aníbal Ept_BR
dc.contributor.authorCastilho, Roger Fpt_BR
unicamp.authorTiago R Figueira, Faculdade de Ciências Médicas, Departamento de Patologia Clínica, Universidade Estadual de Campinas, Campinas, SP 13083-887, Brazil.pt_BR
unicamp.author.externalDaniela R Melo,pt
unicamp.author.externalAníbal E Vercesi,pt
unicamp.author.externalRoger F Castilho,pt
dc.subjectAnimalspt_BR
dc.subjectDigitoninpt_BR
dc.subjectFemalept_BR
dc.subjectFluorescent Dyespt_BR
dc.subjectIndicators And Reagentspt_BR
dc.subjectMembrane Potential, Mitochondrialpt_BR
dc.subjectMitochondria, Liverpt_BR
dc.subjectMitochondrial Proteinspt_BR
dc.subjectMitochondrial Sizept_BR
dc.subjectOrganellespt_BR
dc.subjectPc12 Cellspt_BR
dc.subjectPermeabilitypt_BR
dc.subjectPhenazinespt_BR
dc.subjectRatspt_BR
dc.subjectRats, Wistarpt_BR
dc.description.abstractThe mitochondrial electrical membrane potential (Δψ) is the main component of the proton motive force (Δp) generated across the inner mitochondrial membrane during electron flow through the respiratory chain. Among the techniques available to assess Δψ, methods that rely on the spectrophotofluorometric responses of dyes are widely employed for whole suspensions of isolated mitochondria or permeabilized cells. Safranine is one of the dyes currently used most often for this purpose. Safranine is a lipophilic cationic dye that undergoes optical shifts upon its potential-dependent distribution between the external medium and the intramitochondrial compartment and on its stacking to inner mitochondrial membrane anionic sites. The association between the optical changes of safranine and the membrane potential allows unknown Δψ values to be estimated from an equation describing their relationship. Here, we describe the use of safranine as a fluorescent indicator of Δψ in isolated mitochondria and digitonin-permeabilized cells. We present suitable conditions to employ safranine as a Δψ indicator.en
dc.relation.ispartofMethods In Molecular Biology (clifton, N.j.)pt_BR
dc.relation.ispartofabbreviationMethods Mol. Biol.pt_BR
dc.date.issued2012pt_BR
dc.identifier.citationMethods In Molecular Biology (clifton, N.j.). v. 810, p. 103-17, 2012.pt_BR
dc.language.isoengpt_BR
dc.description.volume810pt_BR
dc.description.firstpage103-17pt_BR
dc.rightsfechadopt_BR
dc.rights.holderpt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1940-6029pt_BR
dc.identifier.doi10.1007/978-1-61779-382-0_7pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/22057563pt_BR
dc.date.available2015-11-27T13:29:10Z-
dc.date.accessioned2015-11-27T13:29:10Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:29:10Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200344-
dc.identifier.idPubmed22057563pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

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