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Type: Artigo de periódico
Title: Quantitative Changes In Human Epithelial Cancers And Osteogenesis Imperfecta Disease Detected Using Nonlinear Multicontrast Microscopy.
Author: Adur, Javier
Pelegati, Vitor B
de Thomaz, Andre A
D'Souza-Li, Lilia
Assunção, Maria do Carmo
Bottcher-Luiz, Fátima
Andrade, Liliana A L A
Cesar, Carlos L
Abstract: We show that combined multimodal nonlinear optical (NLO) microscopies, including two-photon excitation fluorescence, second-harmonic generation (SHG), third harmonic generation, and fluorescence lifetime imaging microscopy (FLIM) can be used to detect morphological and metabolic changes associated with stroma and epithelial transformation during the progression of cancer and osteogenesis imperfecta (OI) disease. NLO microscopes provide complementary information about tissue microstructure, showing distinctive patterns for different types of human breast cancer, mucinous ovarian tumors, and skin dermis of patients with OI. Using a set of scoring methods (anisotropy, correlation, uniformity, entropy, and lifetime components), we found significant differences in the content, distribution and organization of collagen fibrils in the stroma of breast and ovary as well as in the dermis of skin. We suggest that our results provide a framework for using NLO techniques as a clinical diagnostic tool for human cancer and OI. We further suggest that the SHG and FLIM metrics described could be applied to other connective or epithelial tissue disorders that are characterized by abnormal cells proliferation and collagen assembly.
Subject: Adult
Breast Neoplasms
Microscopy, Fluorescence, Multiphoton
Middle Aged
Neoplasms, Glandular And Epithelial
Nonlinear Dynamics
Osteogenesis Imperfecta
Precancerous Conditions
Reproducibility Of Results
Sensitivity And Specificity
Rights: aberto
Identifier DOI: 10.1117/1.JBO.17.8.081407
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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