Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200240
Type: Artigo de periódico
Title: Micrornas Associated With Drought Response In The Bioenergy Crop Sugarcane (saccharum Spp.).
Author: Ferreira, Thaís Helena
Gentile, Agustina
Vilela, Romel Duarte
Costa, Gustavo Gilson Lacerda
Dias, Lara Isys
Endres, Laurício
Menossi, Marcelo
Abstract: Sugarcane (Saccharum spp.) is one of the most important crops in the world. Drought stress is a major abiotic stress factor that significantly reduces sugarcane yields. However the gene network that mediates plant responses to water stress remains largely unknown in several crop species. Although several microRNAs that mediate post-transcriptional regulation during water stress have been described in other species, the role of the sugarcane microRNAs during drought stress has not been studied. The objective of this work was to identify sugarcane miRNAs that are differentially expressed under drought stress and to correlate this expression with the behavior of two sugarcane cultivars with different drought tolerances. The sugarcane cultivars RB867515 (higher drought tolerance) and RB855536 (lower drought tolerance) were cultivated in a greenhouse for three months and then subjected to drought for 2, 4, 6 or 8 days. By deep sequencing of small RNAs, we were able to identify 18 miRNA families. Among all of the miRNAs thus identified, seven were differentially expressed during drought. Six of these miRNAs were differentially expressed at two days of stress, and five miRNAs were differentially expressed at four days. The expression levels of five miRNAs (ssp-miR164, ssp-miR394, ssp-miR397, ssp-miR399-seq 1 and miR528) were validated by RT-qPCR (quantitative reverse transcriptase PCR). Six precursors and the targets of the differentially expressed miRNA were predicted using an in silico approach and validated by RT-qPCR; many of these targets may play important roles in drought tolerance. These findings constitute a significant increase in the number of identified miRNAs in sugarcane and contribute to the elucidation of the complex regulatory network that is activated by drought stress.
Subject: Base Composition
Base Sequence
Biofuels
Dehydration
Droughts
Gene Expression Regulation, Plant
High-throughput Nucleotide Sequencing
Inverted Repeat Sequences
Micrornas
Plant Leaves
Plant Proteins
Rna, Plant
Real-time Polymerase Chain Reaction
Saccharum
Sequence Analysis, Rna
Stress, Physiological
Transcriptome
Rights: aberto
Identifier DOI: 10.1371/journal.pone.0046703
Address: http://www.ncbi.nlm.nih.gov/pubmed/23071617
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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