Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200174
Type: Artigo de periódico
Title: In Vivo And In Vitro Leishmania Amazonensis Infection Induces Autophagy In Macrophages.
Author: Cyrino, Larissa Tavares
Araújo, Alexandra Paiva
Joazeiro, Paulo Pinto
Vicente, Cristina Pontes
Giorgio, Selma
Abstract: Autophagy is the primary mechanism of degradation of cellular proteins and at least two functions can be attributed to this biological phenomenon: increased nutrient supply via recycling of the products of autophagy under nutrient starvation; and antimicrobial response involved in the innate immune system. Many microorganisms induce host cell autophagy and it has been proposed as a pathway by which parasites compete with the host cell for limited resources. In this report we provide evidence that the intracellular parasite Leishmania amazonensis induces autophagy in macrophages. Using western blotting, the LC3II protein, a marker of autophagosomes, was detected in cell cultures with a high infection index. Macrophages infected with L. amazonensis were examined by transmission electronic microscopy, which revealed enlarged myelin-like structures typical late autophagosome and autolysosome. Other evidence indicating autophagy was Lysotracker red dye uptake by the macrophages. Autophagy also occurs in the leishmaniasis skin lesions of BALB/c mice, detected by immunohistochemistry with anti-LC3II antibody. In this study, autophagy inhibitor 3-methyladenine (3MA) reduced the infection index, while autophagy inductors, such as rapamycin or starvation, did not alter the infection index in cultivated macrophages, suggesting that one aspect of the role of autophagy could be the provision of nutritive support to the parasite.
Subject: Adenine
Amines
Animals
Autophagy
Blotting, Western
Bone Marrow Cells
Cells, Cultured
Female
Leishmania Mexicana
Leishmaniasis, Cutaneous
Macrophages
Mice
Mice, Inbred Balb C
Mice, Inbred C57bl
Microtubule-associated Proteins
Sirolimus
Staining And Labeling
Rights: fechado
Identifier DOI: 10.1016/j.tice.2012.08.003
Address: http://www.ncbi.nlm.nih.gov/pubmed/22939777
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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