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Type: Artigo de periódico
Title: Inflammatory And Bone Regulators Expression In Murine Macrophages Under Exposure Of Commercial And Experimental Mineral Trioxide Aggregate.
Author: Pereda, G Obando
Fudinaga, A C Cornejo
Beltrán, H Salas
Peroni, L A
Stach-Machado, D
Abstract: Mineral trioxide aggregate (MTA) has been used in a variety of surgical and non-surgical endodontic applications. The aim of this study was to evaluate the gene expression and protein production of TNF-α, IL-1β and IL-6, as well as the gene expression of RANKL and OPG using both commercial and experimental MTA in macrophage cell cultures. Peritoneal macrophage cell culture was performed. Viability, gene expression of cytokines, RANKL and OPG, and protein levels in experimental- and commercial-grey MTA co-cultured with peritoneal macrophages was determined by tryptan blue, real time PCR and ELISA. The expression of TNF-α for both commercial and experimental MTA was higher, while the expression of IL-1β and IL-6 was similar when compared to the negative control. At protein expression level, no differences were observed between the negative control and cements. RANKL did not show a significant improvement in gene expression when compared with the negative control, but OPG expression in cement samples was higher when compared to the negative control. This study suggests that commercial and experimental MTA promotes anti-inflammatory processes, as well as bone healing capacity.
Subject: Aluminum Compounds
Anti-inflammatory Agents
Calcium Compounds
Cells, Cultured
Drug Combinations
Enzyme-linked Immunosorbent Assay
Gene Expression
Macrophages, Peritoneal
Rank Ligand
Rna, Messenger
Real-time Polymerase Chain Reaction
Citation: Australian Dental Journal. v. 57, n. 3, p. 284-91, 2012-Sep.
Rights: fechado
Identifier DOI: 10.1111/j.1834-7819.2012.01701.x
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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