Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200161
Type: Artigo de periódico
Title: Solid-phase Microextraction Combined With Comprehensive Two-dimensional Gas Chromatography For Fatty Acid Profiling Of Cell Wall Phospholipids.
Author: Bogusz, Stanislau
Hantao, Leandro Wang
Braga, Soraia Cristina Gonzaga Neves
de Matos França, Valtenice de Cássia Rodrigues
da Costa, Marcelo Fernandes
Hamer, Russell David
Ventura, Dora Fix
Augusto, Fabio
Abstract: The combination of solid-phase microextraction (SPME) with comprehensive two-dimensional gas chromatography is evaluated here for fatty acid (FA) profiling of the glycerophospholipid fraction from human buccal mucosal cells. A base-catalyzed derivatization reaction selective for polar lipids such as glycerophospholipid was adopted. SPME is compared to a miniaturized liquid-liquid extraction procedure for the isolation of FA methyl esters produced in the derivatization step. The limits of detection and limits of quantitation were calculated for each sample preparation method. Because of its lower values of limits of detection and quantitation, SPME was adopted. The extracted analytes were separated, detected, and quantified by comprehensive two-dimensional gas chromatography with flame ionization detection (FID). The combination of SPME and comprehensive two-dimensional gas chromatography with FID, using a selective derivatization reaction in the preliminary steps, proved to be a simple and fast procedure for FA profiling, and was successfully applied to the analysis of adult human buccal mucosal cells.
Subject: Cell Wall
Chromatography, Gas
Fatty Acids
Glycerophospholipids
Humans
Mouth Mucosa
Solid Phase Microextraction
Rights: fechado
Identifier DOI: 10.1002/jssc.201200256
Address: http://www.ncbi.nlm.nih.gov/pubmed/22907894
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

Files in This Item:
File SizeFormat 
pmed_22907894.pdf567.95 kBAdobe PDFView/Open


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.