Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200158
Type: Artigo de periódico
Title: Reversion Of Hepatic Steatosis By Exercise Training In Obese Mice: The Role Of Sterol Regulatory Element-binding Protein-1c.
Author: Cintra, Dennys E
Ropelle, Eduardo R
Vitto, Marcelo F
Luciano, Thais F
Souza, Daniela R
Engelmann, Julia
Marques, Scherolin O
Lira, Fabio S
de Pinho, Ricardo A
Pauli, Jose R
De Souza, Cláudio T
Abstract: The dysregulation of regulatory element-binding protein-1c (SREBP-1c) is associated with hepatic steatosis. However, effects of exercise on SREBP-1c protein level in liver have not been investigated. Thus, in this study we investigated if reversion of the hepatic steatosis-induced by exercise training is related with levels of SREBP-1c. Mice were divided into two groups: control lean mice (CT), fed on standard rodent chow, and obese mice (HF), fed on a high-fat diet for 2months. After this period obese mice were divided in two groups: obese mice and obese mice submitted to exercise (HF+EXE). The HF+EXE group performed a running program of 50min per day, 5days per week, for 8weeks. Forty-eight hours after the last exercise session, biochemical, immunoblotting, histology and immunohistochemistry analyses were performed. Livers of HF mice showed increased SREBP-1c, FAS (Fatty Acid Synthase), SCD1 (Stearoyl-CoA Desaturase1) and CPT1 (Carnitine Palmitoyl Transferase1) protein levels (3.4, 5.0, 2.6 and 2.9 times, respectively), though ACC (Acetyl-CoA Carboxilase) phosphorylation dropped 4.2 times. In livers of HF+EXE, levels of SREBP-1c, FAS, SCDI and CPTI decreased 2.1, 1.9, 1.8, and 2.7 times, respectively), while ACC phosphorylation increased 3.0 times. Lower SREBP-1c protein levels after exercise were confirmed also by immunohistochemistry. Total liver lipids content was higher in HF (2.2 times) when compared to CT, and exercise training reduced it significantly (1.7 times). Our study allows concluding that the reduction in SREBP-1c protein levels is associated with steatosis reversion induced by exercise training.
Subject: Acetyl-coa Carboxylase
Animals
Carnitine O-palmitoyltransferase
Fatty Acid Synthases
Fatty Liver
Liver
Male
Mice
Mice, Obese
Phosphorylation
Physical Conditioning, Animal
Stearoyl-coa Desaturase
Sterol Regulatory Element Binding Protein 1
Rights: fechado
Identifier DOI: 10.1016/j.lfs.2012.08.002
Address: http://www.ncbi.nlm.nih.gov/pubmed/22906631
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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