Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200151
Type: Artigo de periódico
Title: Electroacupuncture Increases The Concentration And Organization Of Collagen In A Tendon Healing Model In Rats.
Author: de Almeida, Marcos dos Santos
de Aro, Andréa Aparecida
Guerra, Flávia Da Ré
Vieira, Cristiano Pedrozo
de Campos Vidal, Benedicto
Rosa Pimentel, Edson
Abstract: The aim of this study was to investigate the effect of electroacupuncture (EA) on the composition and organization of the extracellular matrix of the rat Achilles tendon after a partial transection during the proliferative phase of healing. Wistar rats were divided into three groups: rats that were not tenotomized (G1), tenotomized rats (G2), and rats that were tenotomized and submitted to EA (G3). EA was applied 15 days after injury at the ST36 and BL57 acupoints for 20 min, three times per week on alternate days for a total of six sessions. Biochemical analyses were performed using non-collagenous proteins, glycosaminoglycans, and hydroxyproline quantifications. An analysis of metalloproteinase-2 was carried out by zymography. The general organization of the extracellular matrix and the metachromasy of the tendons were analyzed under light microscopy. The organization of the bundles of collagen fibers was analyzed by birefringence analysis. The results showed that EA did not alter the concentration of non-collagenous proteins or glycosaminoglycans or the enzymatic activity of metalloproteinase-2 in the transected tendons. However, the concentration of hydroxyproline was significantly increased when these tendons were treated by EA. The analysis of birefringence showed a higher organization of collagen fibers in the group treated by EA. These results indicate, for the first time, that EA may offer therapeutic benefits for the treatment of tendon injuries by increasing the concentration of collagen and by inducing a better molecular organization of the collagen fibers, which may improve the mechanical strength of the tendon after injury.
Subject: Achilles Tendon
Animals
Collagen
Electroacupuncture
Hydroxyproline
Male
Matrix Metalloproteinase 2
Rats
Rats, Wistar
Tendon Injuries
Rights: fechado
Identifier DOI: 10.3109/03008207.2012.710671
Address: http://www.ncbi.nlm.nih.gov/pubmed/22891942
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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