Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200148
Type: Artigo de periódico
Title: Molecular Cloning And Insecticidal Effect Of Inga Laurina Trypsin Inhibitor On Diatraea Saccharalis And Heliothis Virescens.
Author: Ramos, Vanessa da S
Cabrera, Odalys G
Camargo, Eduardo L O
Ambrósio, Alinne B
Vidal, Ramon O
da Silva, Desireé S
Guimarães, Lays C
Marangoni, Sérgio
Parra, José R P
Pereira, Gonçalo A G
Macedo, Maria L R
Abstract: Native Inga laurina (Fabaceae) trypsin inhibitor (ILTI) was tested for anti-insect activity against Diatraea saccharalis and Heliothis virescens larvae. The addition of 0.1% ILTI to the diet of D. saccharalis did not alter larval survival but decreased larval weight by 51%. The H. virescens larvae that were fed a diet containing 0.5% ILTI showed an 84% decrease in weight. ILTI was not digested by the midgut proteinases of either species of larvae. The trypsin levels were reduced by 55.3% in the feces of D. saccharalis and increased by 24.1% in the feces of H. virescens. The trypsin activity in both species fed with ILTI was sensitive to the inhibitor, suggesting that no novel proteinase resistant to ILTI was induced. Additionally, ILTI exhibited inhibitory activity against the proteinases present in the larval midgut of different species of Lepidoptera. The organization of the ilti gene was elucidated by analyzing its corresponding genomic sequence. The recombinant ILTI protein (reILTI) was expressed and purified, and its efficacy was evaluated. Both native ILTI and reILTI exhibited a similar strong inhibitory effect on bovine trypsin activity. These results suggest that ILTI presents insecticidal properties against both insects and may thus be a useful tool in the genetic engineering of plants.
Subject: Animals
Base Sequence
Cloning, Molecular
Enzyme Activation
Enzyme Assays
Escherichia Coli
Fabaceae
Feces
Genes, Plant
Insecticides
Larva
Lepidoptera
Pest Control, Biological
Plant Proteins
Proteolysis
Recombinant Fusion Proteins
Seeds
Trypsin Inhibitors
Weight Loss
Rights: fechado
Identifier DOI: 10.1016/j.cbpc.2012.07.007
Address: http://www.ncbi.nlm.nih.gov/pubmed/22885277
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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