Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200146
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dc.contributor.CRUESPUNIVERSIDADE DE ESTADUAL DE CAMPINASpt_BR
dc.typeArtigo de periódicopt_BR
dc.titleMechanical And Biological Characterization Of Resin-modified Glass-ionomer Cement Containing Doxycycline Hyclate.pt_BR
dc.contributor.authorde Castilho, Aline R Fpt_BR
dc.contributor.authorDuque, Cristianept_BR
dc.contributor.authorNegrini, Thais de Cpt_BR
dc.contributor.authorSacono, Nancy Tpt_BR
dc.contributor.authorde Paula, Andréia Bpt_BR
dc.contributor.authorSacramento, Patrícia Apt_BR
dc.contributor.authorde Souza Costa, Carlos Apt_BR
dc.contributor.authorSpolidorio, Denise M Ppt_BR
dc.contributor.authorPuppin-Rontani, Regina Mpt_BR
unicamp.authorAline R F de Castilho, Department of Pediatric Dentistry, Piracicaba Dental School, University of Campinas (UNICAMP), Piracicaba, São Paulo, Brazil.pt_BR
unicamp.author.externalCristiane Duque,pt
unicamp.author.externalThais de C Negrini,pt
unicamp.author.externalNancy T Sacono,pt
unicamp.author.externalAndréia B de Paula,pt
unicamp.author.externalPatrícia A Sacramento,pt
unicamp.author.externalCarlos A de Souza Costa,pt
unicamp.author.externalDenise M P Spolidorio,pt
unicamp.author.externalRegina M Puppin-Rontani,pt
dc.subjectAnti-bacterial Agentspt_BR
dc.subjectBiological Assaypt_BR
dc.subjectCell Linept_BR
dc.subjectColony Count, Microbialpt_BR
dc.subjectDental Cariespt_BR
dc.subjectDoxycyclinept_BR
dc.subjectGlass Ionomer Cementspt_BR
dc.subjectMicrobial Sensitivity Testspt_BR
dc.subjectResin Cementspt_BR
dc.description.abstractTo characterize the mechanical and biological properties of a resin-modified glass ionomer cement (RMGIC) containing doxycycline hyclate. The antibacterial effect of RMGIC containing 1.5, 3.0 and 4.5% doxycycline hyclate was assessed using two experiments - agar diffusion test for 24h and biofilm assay for 24h and 7 days - against some cariogenic bacteria. Briefly, base layers of BHI agar and 300μL of each inoculum were prepared in Petri dishes with 6 wells that were completely filled with materials. After 24h incubation, zones of bacterial growth inhibition were measured using a digital caliper. Biofilm assays were conducted using RMGIC specimens immersed in 24-well plates containing the inoculum in BHI broth. After 24h and 7 days, each specimen were removed, vortexed and the suspension diluted and inoculated in BHI plates for subsequent bacterial counting. Cytotoxicity tests used 50 specimens made in sterilized metal molds, including Vitrebond as positive control. Extracts from every specimen were applied on the MDPC-23 odontoblast-like cells for 24h. The MTT assay and SEM evaluation determined cell metabolism and morphology, respectively. 80 cylindrical specimens were made from the previously cited groups, and were submitted to testing with a universal testing machine (Instron 4411) using a crosshead speed of 1.0mm/min for compressive strength and 0.5mm/min for diametral tensile strength, respectively. Data from antibacterial and cytotoxic effects, and mechanical properties were submitted to appropriated statistical tests. All tested groups showed growth inhibition of all tested strains (p<0.05) in 24h for both microbiological tests, but only 4.5% doxycycline have antibacterial effect after 7 days. None of doxycycline concentrations caused toxic effect to the MDPC-23 cells or presenting alterations to mechanical properties. The incorporation of up to 4.5% doxycycline hyclate into RMGIC inhibits important oral microorganisms, without modifying biological and mechanical characteristics of the dental material, suggesting a new alternative for the treatment of dental caries.en
dc.relation.ispartofArchives Of Oral Biologypt_BR
dc.relation.ispartofabbreviationArch. Oral Biol.pt_BR
dc.date.issued2012-Febpt_BR
dc.identifier.citationArchives Of Oral Biology. v. 57, n. 2, p. 131-8, 2012-Feb.pt_BR
dc.language.isoengpt_BR
dc.description.volume57pt_BR
dc.description.firstpage131-8pt_BR
dc.rightsfechadopt_BR
dc.rights.holderCopyright © 2011 Elsevier Ltd. All rights reserved.pt_BR
dc.sourcePubMedpt_BR
dc.identifier.issn1879-1506pt_BR
dc.identifier.doi10.1016/j.archoralbio.2011.08.009pt_BR
dc.identifier.urlhttp://www.ncbi.nlm.nih.gov/pubmed/21920494pt_BR
dc.date.available2015-11-27T13:28:48Z-
dc.date.accessioned2015-11-27T13:28:48Z-
dc.description.provenanceMade available in DSpace on 2015-11-27T13:28:48Z (GMT). No. of bitstreams: 0 Previous issue date: 2012en
dc.identifier.urihttp://repositorio.unicamp.br/jspui/handle/REPOSIP/200146-
dc.identifier.idPubmed21920494pt_BR
Appears in Collections:Unicamp - Artigos e Outros Documentos

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