Please use this identifier to cite or link to this item: http://repositorio.unicamp.br/jspui/handle/REPOSIP/200125
Type: Artigo de periódico
Title: Polyploidy And Chromatin Remodeling In Hepatocytes From Insulin-dependent Diabetic And Normoglycemic Aged Mice.
Author: Ghiraldini, Flávia G
Silva, Isabela S
Mello, Maria Luiza S
Abstract: Changes in polyploidization, chromatin supraorganization, and chromatin accessibility were investigated in hepatocytes collected from adult, nonobese diabetic (NOD) mice with increasing hyperglycemia and compared with adult normoglycemic controls and 56-week-old normoglycemic BALB/c mice. Our goal was to determine the changes in ploidy degrees and chromatin characteristics in mouse hepatocytes that are associated with insulin-dependent diabetes and to detect similarities in these aspects with those verified with aging, with greater accuracy than previous studies. Image analysis of Feulgen-stained nuclei revealed changes in ploidy degrees and chromatin supraorganization. Chromatin accessibility was assessed with micrococcal nuclease (MNase) digestion. Increased polyploidy was associated with increasing levels of glycemia, and this trend toward polyploidy was found even under normoglycemic conditions in NOD mice. Although high degrees of ploidy were also detected in aged BALB/c mice, the magnitude of polyploidy was not the same magnitude as that in the diabetic mice. While there was increased homogeneity of chromatin packaging with increasing polyploidy under conditions of severe hyperglycemia (and even under conditions of normoglycemia) in NOD mice, an inverse relationship was observed in aged BALB/c mice. Chromatin accessibility to MNase increased under severe hyperglycemia and advanced age, but it was much higher in the diabetic mice. In conclusion, although similarities in polyploidy were observed between the hepatocytes from increasingly hyperglycemic adult mice and those from normoglycemic aged mice, the relationship between chromatin remodeling and increases in ploidy degrees was not the same between the hepatocytes of these two groups. These findings demonstrate that strict similarities between diabetes and aging are not always true at the cellular level. This discordance is likely due to differences in the metabolic state of mouse hepatocytes during aging and diabetic conditions consequent to specificities in their gene regulatory programs.
Subject: Aging
Animals
Blood Glucose
Chromatin
Chromatin Assembly And Disassembly
Coloring Agents
Dna
Dna Cleavage
Diabetes Mellitus, Type 1
Female
Hepatocytes
Image Processing, Computer-assisted
Mice
Mice, Inbred Balb C
Mice, Inbred Nod
Micrococcal Nuclease
Polyploidy
Rosaniline Dyes
Rights: fechado
Identifier DOI: 10.1002/cyto.a.22102
Address: http://www.ncbi.nlm.nih.gov/pubmed/22837107
Date Issue: 2012
Appears in Collections:Artigos e Materiais de Revistas Científicas - Unicamp

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