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Type: Artigo de periódico
Title: Comparison Of Endotoxin Levels Found In Primary And Secondary Endodontic Infections.
Author: Gomes, Brenda P F A
Endo, Marcos S
Martinho, Frederico C
Abstract: This clinical study was conducted to compare the levels of endotoxins (lipopolysaccharides [LPSs]) found in primary and secondary endodontic infections with apical periodontitis by correlating LPS contents with clinical/radiographic findings. In addition, the presence of target gram-negative anaerobic bacteria was also investigated. Samples were taken from 15 root canals with primary infections and 15 with secondary infections by using paper points. The limulus amebocyte lysate assay was used to quantify endotoxins, and the polymerase chain reaction technique (16S rDNA) was used for bacterial investigation. Endotoxins were detected in 100% of the root canal samples collected from primary (15/15) and secondary (15/15) infections with median values of 7.49 EU/mL and 3.96 EU/mL, respectively (P < .05). The median value of endotoxins found in the presence of clinical symptoms was significantly higher than in asymptomatic teeth with primary infections (P < .05). A positive correlation was found between endotoxin contents and a larger size of the radiolucent area (>3 mm) (P < .05). Prevotella nigrescens (10/15, 4/15), Fusobacterium nucleatum (5/15, 1/15), Treponema denticola (3/15, 1/15), and Treponema socranskii (5/15, 1/15) were detected in teeth with primary and secondary infections, respectively. P. endodontalis was present only in teeth with primary infections (5/15). Teeth with primary endodontic infections had higher contents of endotoxins and a more complex gram-negative bacterial community than teeth with secondary infections. Moreover, the levels of endotoxins were related to the severity of bone destruction in periapical tissues as well as the development of clinical features in teeth with primary infections.
Subject: Asymptomatic Diseases
Dna, Bacterial
Dental Pulp Cavity
Dental Pulp Diseases
Dental Pulp Necrosis
Fusobacterium Infections
Fusobacterium Nucleatum
Gram-negative Bacterial Infections
Limulus Test
Pain Measurement
Periapical Periodontitis
Polymerase Chain Reaction
Porphyromonas Endodontalis
Prevotella Nigrescens
Treponema Denticola
Treponemal Infections
Citation: Journal Of Endodontics. v. 38, n. 8, p. 1082-6, 2012-Aug.
Rights: fechado
Identifier DOI: 10.1016/j.joen.2012.04.021
Date Issue: 2012
Appears in Collections:Unicamp - Artigos e Outros Documentos

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